Register for the Latest CYTO U Webinar: "Kinetic Image Cytometry of Stem Cell-Derived Cardiomyocytes and Neurons" by Jeffrey Price

[Pulliam, Kanika F.]

Kinetic Image Cytometry of Stem Cell-Derived Cardiomyocytes and Neurons



Presented by

Jeffrey Price, M.D., Ph.D.

Professor, Scintillon Institute

CEO, Vala Sciences

Adjunct Professor, Sanford-Burnham-Prebys Medical Discovery Institute



July 28, 2015

9 am PT/Canada
12:00 pm ET US/Canada
5:00 pm GMT/UK/Portugal

Register: http://cytou.peachnewmedia.com
Cost: FREE

Jeff Price has dedicated his scientific career to creating new engineering technologies to improve human health, mostly focused on tools for early drug discovery and pathology diagnostics. This includes fundamental aspects of instrumentation such as increasing speed and resolution in high content screening (HCS) instrumentation as well as designing fundamentally new approaches such as kinetic image cytometry (KIC) for obtaining more informative physiological data from living "human-in-a-dish" culture models derived from induced pluripotent stem cell cell-derived cardiomyocytes and neurons. His medical-scientific passion arose from first obtaining an M.D. from Loma Linda University in 1985 and then a Ph.D. in Bioengineering from UCSD in 1990. After building the first prototype high content screening (HCS) / image cytometry instrument for his dissertation in 1990, he obtained independent funding first from the Whitaker Foundation and then NSF and NIH, advanced the instrumentation, received six patents, and founded Q3DM, Inc., in 1998. Q3DM named the instrument the EIDAQ 100, and Beckman Coulter purchased Q3DM in 2003 and renamed the instrument the IC 100. In 2004, Dr. Price co-founded Vala Sciences Inc. and joined the Sanford-Burnham-Prebys Medical Discovery Institute (SBP) as associate professor. In 2005, Vala Sciences, in collaboration with the SBP labs of Drs. Price and Mercola, obtained the first NIH funding for KIC and built the first prototype. In 2014, Dr. Price joined the Scintillon Institute as professor.


Webinar Summary:

Kinetic image cytometry (KIC) creates single cell measurements of dynamics of events such as action potentials and calcium transients in all cells, and is especially compelling for fast-acting cells such as cardiomyocytes and neurons. Kinetic plate readers (e.g., FLIPR and FDSS) provide well-averaged readouts that require synchronized cellular behaviors, whereas our novel KIC parameterizes Ca2+ transients and action potentials on each cell; e.g., typically on 300-500 cardiomyocytes in a field of view simultaneously. Developed collaboratively by Vala Sciences and Jeff Price's and Mark Mercola's academic labs at Sanford-Burnham-Prebys Medical Discovery Institute (SBP), KIC has been used to detect arrhythmogenic and toxic effects in early drug discovery, and for drug discovery for heart failure. The presentation will primarily report on the accuracy of KIC for predicting arrhythmia from kinetics of the calcium transients in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), and will also include some examples of application to hiPSC-derived neurons.
CYTO U Webinar Recordings
A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all.  You may also want to watch the recordings of CYTO U's last three webinars, Growing a Successful and Fruitful Core: You Reap What You Sow by Joanne Lannigan, The Use of Nanoparticles for Cell Identification and Tracking by Paul Rees, and Predicting the Best Resolution and Sensitivity in Panel Development and Reducing Inter-instrument Variability in Flow Cytometry by Steve Perfetto and Jim Wood at CYTO U.  For more details, visit: http://cytou.peachnewmedia.com.

CYTO University
CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, and interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all. Learn more at http://cytou.peachnewmedia.com

CYTO U Access for ISAC Members
For current ISAC members, your user login and password to CYTO U are the same user login and password you have for the members-only section of the ISAC Web site. If you remember your user log in to the ISAC Web site but cannot remember your password, you can obtain your password from the ISAC Web site by clicking on the link "forgot your password?".  If you cannot remember your username, you can contact [hidden email]<mailto:[hidden email]> for your login information.


____________________
Kanika F. Pulliam, Ph.D.
Education Manager
International Society for Advancement of Cytometry (ISAC)
9650 Rockville Pike
Bethesda, MD 20814
[phone]
[hidden email]<mailto:[hidden email]>


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