Reverse density/intensity measurement

[Dipanjana Ghosh]

Dear all,
I was using mostly confocal images to measure intensities where the signal
comes as bright and the background is dark.
Now when I am opening a western blot image where the signal is dark and
background is bright, it seems the software is still calculating as it does
for the confocal images. What to amend in the set measurement or some other
settings so that it can take care of respective dark or bright signals?
I Hope I can explain my problem. If not please feel free to ask questions.

Thanking you

Regards,
Dipanjana

--
Dr. Dipanjana Ghosh

Prev: Post doctoral Research Fellow
Department of Biological Sciences
National University of Singapore
Singapore

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[CARL Philippe (LBP)]

Dear Dipanjana,
Please apologize in the case I'm not correctly understanding your question.
But simply doing Edit>Invert (or Ctrl + Shift + I) in order to invert your image won't it be enough to solve your issue?
My best regards,
Philippe

Philippe CARL
Laboratoire de Bioimagerie et Pathologies
UMR 7021 CNRS - Université de Strasbourg
Faculté de Pharmacie
74 route du Rhin
67401 ILLKIRCH
Tel : [phone]

----- Mail original -----
De: "Dipanjana Ghosh" <[hidden email]>
À: "imagej" <[hidden email]>
Envoyé: Lundi 18 Janvier 2021 09:17:58
Objet: Reverse density/intensity measurement

Dear all,
I was using mostly confocal images to measure intensities where the signal
comes as bright and the background is dark.
Now when I am opening a western blot image where the signal is dark and
background is bright, it seems the software is still calculating as it does
for the confocal images. What to amend in the set measurement or some other
settings so that it can take care of respective dark or bright signals?
I Hope I can explain my problem. If not please feel free to ask questions.

Thanking you

Regards,
Dipanjana

--
Dr. Dipanjana Ghosh

Prev: Post doctoral Research Fellow
Department of Biological Sciences
National University of Singapore
Singapore

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Dipanjana Ghosh]

Dear Phillippe,

It worked this way. Thanks a lot.

Regards,
Dipanjana

On Mon, 18 Jan 2021, 19:00 CARL Philippe (LBP), <[hidden email]>
wrote:

> Dear Dipanjana,
> Please apologize in the case I'm not correctly understanding your question.
> But simply doing Edit>Invert (or Ctrl + Shift + I) in order to invert your
> image won't it be enough to solve your issue?
> My best regards,
> Philippe
>
> Philippe CARL
> Laboratoire de Bioimagerie et Pathologies
> UMR 7021 CNRS - Université de Strasbourg
> Faculté de Pharmacie
> 74 route du Rhin
> 67401 ILLKIRCH
> Tel : [phone]
>
> ----- Mail original -----
> De: "Dipanjana Ghosh" <[hidden email]>
> À: "imagej" <[hidden email]>
> Envoyé: Lundi 18 Janvier 2021 09:17:58
> Objet: Reverse density/intensity measurement
>
> Dear all,
> I was using mostly confocal images to measure intensities where the signal
> comes as bright and the background is dark.
> Now when I am opening a western blot image where the signal is dark and
> background is bright, it seems the software is still calculating as it does
> for the confocal images. What to amend in the set measurement or some other
> settings so that it can take care of respective dark or bright signals?
> I Hope I can explain my problem. If not please feel free to ask questions.
>
> Thanking you
>
> Regards,
> Dipanjana
>
> --
> Dr. Dipanjana Ghosh
>
> Prev: Post doctoral Research Fellow
> Department of Biological Sciences
> National University of Singapore
> Singapore
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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