A great description of the rolling ball background subtraction is in the
documentation:
http://rsb.info.nih.gov/ij/docs/menus/process.html#backgroundThe ball size should be of a diameter that it wouldn't 'fall in' to one of
your objects of interest if it was rolling along that line in the example
(but inverted). If you choose something too small it will remove all but
the highest peaks and the aim of the tool is just to make a 'level playing
field'. Sometimes for 'background' or non-interesting objects that are
large but of lower intensity it will just take the edges of them.
Brooke Kelley
On Wed, 28 Jun 2006, Thomas Sadowski wrote:
> Hello all,
>
>
> I have been using the background subtraction to clean up allot of the noise in many of my images. We have played around with many radii and come to the conclusion that the default of 50 performs the best. Does anyone have any explanation as to why this is??? Why is is that the default of 50 works so well in comparison with other radii??
>
>
> Thank you in advance,
>
> Thomas Sadowski
> Southern Connecticut State University
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