SPE movies

[Luciano Marpegan]

Hello everybody, I'm trying to open SPE movie files with the openSPE plugin
but it only opens the first frame. So what I'm doing now is splitting the
movie into single .spe images with the winview software and then import
them as an image sequence with imageJ. But I would prefer to do everything
using imageJ. I know a similar question was posted in November 2004 but I
couldn't find any answer to it in the archives. I hope someone has an
answer now.

Any help will be really appreciated.

Best,
Luciano.

Biology Department,
Washington University in St. Louis, USA  

[Brooke Kelley]

In Winview you can save the .spe file as a 16 bit .tif file.  ImageJ will
open this as a stack.  You can then split the stack into individual images
in ImageJ if you like.

Brooke Kelley


On Fri, 16 Sep 2005, Luciano Marpegan wrote:

> Hello everybody, I'm trying to open SPE movie files with the openSPE
> plugin but it only opens the first frame. So what I'm doing now is
> splitting the movie into single .spe images with the winview software and
> then import them as an image sequence with imageJ. But I would prefer to
> do everything using imageJ. I know a similar question was posted in
> November 2004 but I couldn't find any answer to it in the archives. I hope
> someone has an answer now.
>
> Any help will be really appreciated.
>
> Best,
> Luciano.
>
> Biology Department,
> Washington University in St. Louis, USA

[Luciano Marpegan]

Brooke, thank you so much for your suggestion. It worked perfect.

Luciano Marpegan


At 05:34 PM 9/16/2005, you wrote:

>In Winview you can save the .spe file as a 16 bit .tif file.  ImageJ will
>open this as a stack.  You can then split the stack into individual images
>in ImageJ if you like.
>
>Brooke Kelley
>
>On Fri, 16 Sep 2005, Luciano Marpegan wrote:
>
> > Hello everybody, I'm trying to open SPE movie files with the openSPE
> > plugin but it only opens the first frame. So what I'm doing now is
> > splitting the movie into single .spe images with the winview software and
> > then import them as an image sequence with imageJ. But I would prefer to
> > do everything using imageJ. I know a similar question was posted in
> > November 2004 but I couldn't find any answer to it in the archives. I hope
> > someone has an answer now.
> >
> > Any help will be really appreciated.
> >
> > Best,
> > Luciano.
> >
> > Biology Department,
> > Washington University in St. Louis, USA