Johannes Schindelin wrote
Hi,
On Tue, 28 Jul 2009, Benedikt Nimmervoll wrote:
> I am looking for a plugin that allows me to select a certain number of
> the brightest pixels surrounding the pixel I am clicking on.
That is very easy to code as a macro.
> Circularity should, if possible, also be a selection criteria.
How can pixels be circular? Please clarify.
The selected regions should preferably be circular. This is not a main criteria for the plugin/macro since the morphology of the cells should take care of the circular shape of the regions anyway
Johannes Schindelin wrote
> In further steps I want to transfer the selected area to a ROI and use
> this ROI for intensity measurements in a stack (these functions do not
> need to be included in the plugin, this is just for your information).
Selected areas _are_ ROIs.
But now you got me thoroughly confused: you want to select pixels, yet you
talk about selected areas. That does not parse with this developer.
Some procedures are using treshold masks (e.g. this manual for
particle analysis ), which requires an additional step on the way to the final ROI which can e.g be used with the ROI manager.
The selected bright pixels should be neighbouring the pixel I clicked on. Therefore in sum the selected pixels should merge to an area/region.
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