Dear Kees,
Thank you for your help. It worked perfectly. I have read and played and
played but should have asked this question a long time ago.
Appreciate your help.
Regards
Simon
Dr Simon Locke
MBBS BMed Sci FACSP FFSEM (UK) FRACGP
Sport and Exercise Physician
Bendigo Orthopaedic and Sports Medicine Clinic
176 Barnard st, Bendigo, Victoria, 3550
P:613 5442 5288 F:613 5442 5399 W:sportmovementcentre.com
Centre for Sport+ Movement is now on Facebook
http://www.facebook.com-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Straatman, Kees (Dr.)
Sent: Sunday, 8 December 2013 10:52 PM
To:
[hidden email]
Subject: Re: Selecting and measuring bvs
Dear Simon
If you split your channels (Image> Color > Split Channels) and subtract the
blue channel from the red (Process > Image Calculator and select the proper
images) you get an image with only your red areas. So these can easily be
selected via threshold and added to the ROI manager (via Analyze>Analyze
particles (set limits if necessary and flag Add to Manager). This will give
your red selection.
Yes, you can Image properties for this or Analyze > Set Scale.
Best wishes
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
University of Leicester
http://www.le.ac.uk/biochem/microscopy/home.html-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Simon
Locke
Sent: 08 December 2013 04:24
To:
[hidden email]
Subject: Selecting and measuring bvs
Hi
I am trying to measure the Bvs shown above. They represent "neovascularity"
in symptomatic tendons. I look for the maximum that occurs with the pulse.
Is there a way to automate "red" area selection? I can leave it in a RGB
format.
Is there an alternative to free hand selections and using ROI manager?
Unfortunately, I am not familiar with writing JAVA script and am limited to
tools.
When I change the area from pixels to cms, do I just change cm in image
properties?
Thank you in anticipation. I apologise for being limited in developing but
I am foremost a clinician.
Dr Simon Locke
MBBS BMed Sci FACSP FFSEM (UK) FRACGP
Sport and Exercise Physician
Bendigo Orthopaedic and Sports Medicine Clinic
176 Barnard st, Bendigo, Victoria, 3550
P:613 5442 5288 F:613 5442 5399 W:sportmovementcentre.com
Centre for Sport+ Movement is now on Facebook
http://www.facebook.com
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