Serial Macro Extensions, eslide, and Ludl microscope stage

[Smiley, John]

Dear All:
I have been controlling Ludl microscope stage motors using "eslide" and ImageJ.   However, while this works with my older Ludl controllers (Mac 2000/Mac 2002), I am unable to establish serial communication with a newer Mac 5000.  This appears to be an issue with eslide, because I can establish communication using "hyperterminal" software.
Has anyone else overcome this problem?  I contacted the creators of eslide, who were very helpful in the past, but it seems they no longer support the software.
An alternative solution would be to use the "Serial Macro Extensions" plugin.   However, my initial attempts to communicate with Ludl controllers were not successful, and I am unable to find much in the way of documentation.   Is there is anyone who has done this with a Ludl or similar stage?  An example serial communication would be very helpful.

Thanks,
John Smiley


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[Collin Poczatek]

Hi John,

The Ludl Mac 5000 works great with Micro-Manager (we have one).

Quote:
"?Manager is a software package for control of automated microscopes.
Together with the image processing application ImageJ, ?Manager provides
a comprehensive, freely available, imaging solution.""

http://valelab.ucsf.edu/~MM/MMwiki/ <http://valelab.ucsf.edu/%7EMM/MMwiki/>
http://valelab.ucsf.edu/~MM/MMwiki/index.php/Ludl 
<http://valelab.ucsf.edu/%7EMM/MMwiki/index.php/Ludl>
http://valelab.ucsf.edu/~MM/MMwiki/index.php/Script_Panel_GUI 
<http://valelab.ucsf.edu/%7EMM/MMwiki/index.php/Script_Panel_GUI>

Not sure what eslide is/was/does, but hopefully this helps.

Collin


On 06/05/2013 12:00 PM, Smiley, John wrote:

> Dear All:
> I have been controlling Ludl microscope stage motors using "eslide" and ImageJ.   However, while this works with my older Ludl controllers (Mac 2000/Mac 2002), I am unable to establish serial communication with a newer Mac 5000.  This appears to be an issue with eslide, because I can establish communication using "hyperterminal" software.
> Has anyone else overcome this problem?  I contacted the creators of eslide, who were very helpful in the past, but it seems they no longer support the software.
> An alternative solution would be to use the "Serial Macro Extensions" plugin.   However, my initial attempts to communicate with Ludl controllers were not successful, and I am unable to find much in the way of documentation.   Is there is anyone who has done this with a Ludl or similar stage?  An example serial communication would be very helpful.
>
> Thanks,
> John Smiley
>
>
> ________________________________
> Conserve Resources. Print only when necessary.
>
> IMPORTANT NOTICE: This e-mail is meant only for the use of the intended recipient. It may contain confidential information which is legally privilegedor otherwise protected by law. If you received this e-mail in error or from someone who is not authorized to send it to you, you are strictly prohibited from reviewing, using, disseminating, distributing or copying the e-mail. PLEASE NOTIFY US IMMEDIATELY OF THE ERROR BY RETURN E-MAIL AND DELETE THIS MESSAGE FROM YOUR SYSTEM. Thank you for your cooperation.
>
> IMPORTANT NOTICE: This e-mail is meant only for the use of the intended recipient. It may contain confidential information which is legally privileged or otherwise protected by law. If you received this e-mail in error or from someone who is not authorized to send it to you, you are strictly prohibited from reviewing, using, disseminating, distributing or copying the e-mail. PLEASE NOTIFY US IMMEDIATELY OF THE ERROR BY RETURN E-MAIL AND DELETE THIS MESSAGE FROM YOUR SYSTEM. Thank you for your cooperation.
>
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[ALEMBIC | Cesare]

Dear List,
I am looking for tips on how to good quantify a sort of cell interactions in a time-lapse experiment.

Very briefly:
we have some movies with different fluorescent cells, i.e green and red.
These two cell types move in the field of view and occasionally interact each other and thus remain "bonded" for some time.

We would like to quantify how many cells green&red stay very close (let's say "touching") and for how long time (i.e number of frame).
Is there an easy way to do or an existing plugin that allows such measurements?

Thanks in advance!

Cesare






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