Hi Stéphanie,
You can fix it if you uncheck the first box and check the second box in that pop-up message.
In other words, you are telling ImageJ that you want to 1) keep the global calibration and 2) stop getting the error message. Subsequent images will open with your desired calibration.
There is a risk with this: if you then open an image with a different magnification, it will erroneously be set to the calibration you established for the first image. So you have to be careful to re-set the scale or Remove Scale if you change to another dataset at a different magnification.
Hope this helps.
Theresa
On Dec 16, 2014, at 2:22 PM, Stephanie Bourgon <
[hidden email]<mailto:
[hidden email]>> wrote:
Hi everyone,
I have been trying to set the scale for my analysis and I am experiencing problems. I open my image and set the scale like it was shown in the tutorial (distance in pixel measured with line, known distance, pixel aspect ratio, and unit of lenght) then I check global because I have many images at the same magnification to analyze. However everytime I try to open a new image this message pops up:
The calibration of this image conflicts with the current global calibration.
-Disable global calibration
-Disable these messages
Does someone know how I can fix this? I have over 6000 images to analyze and I have no idea what I am doing wrong.
Thank you,
Stéphanie Bourgon
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Theresa Swayne, Ph.D.
Manager
Confocal and Specialized Microscopy Shared Resource<
http://hiccc.columbia.edu/research/sharedresources/confocal>
Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
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Phone: 212-851-4613
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