Single molecule photobleaching

[rosnies]

Hi all,

I was wondering if anyone has used Image J to analyze TIRF image stacks of single molecule photobleaching. I want to look at the step-wise bleaching of membrane proteins tagged with a fluorescent protein. I would need to first identify fluorescent spots on the first frame and then look at the fluorescent intensity changes of these spots over time. I understand that the signal is quite noisy and needs some filtering to be able to visualize the discrete bleaching steps. Others have used the 'Chung–Kennedy algorithm' to remove statistical noise and reveal photobleaching steps.  

Does anyone know of a plugin that could be used for this purpose?

Thanks,
Rose