Spherical aberration in Electron photomicrographs

[Porter, George M.D.]

Hello,

I am trying to stitch together montages of cardiac myocytes from electron micrographs and I find what I believe to be spherical aberration in the images (which apparently can happen with a side mount camera). Is there a way to correct this aberration in the images electronically using ImageJ or any other program? I have tried using the Warp Transformation and Lens Correction and Adaptive Wild Angle Filters in Photoshop without consistent success.

Thanks,
George Porter

George A. Porter, Jr., MD, PhD
Professor
Chief and Fellowship Director, Division of Cardiology
Department of Pediatrics
University of Rochester Medical Center
601 Elmwood Ave.
Box 631
Rochester, NY  14642
Phone: [phone]
Fax: [phone]
Email: [hidden email]

"Let me say as I've always said, and I will always continue to say, that riots are socially destructive and self-defeating. ... But in the final analysis, a riot is the language of the unheard. And what is it that America has failed to hear? It has failed to hear that the plight of the Negro poor has worsened over the last few years. It has failed to hear that the promises of freedom and justice have not been met. And it has failed to hear that large segments of white society are more concerned about tranquility and the status quo than about justice, equality, and humanity. And so in a real sense our nation's summers of riots are caused by our nation's winters of delay. And as long as America postpones justice, we stand in the position of having these recurrences of violence and riots over and over again."
Martin Luther King, Jr., "The Other America"


--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Saalfeld, Stephan]

Can you acquire additional images of textured samples on the same
system or do you already have sufficient overlap (>50%)?

Then you could use this protocol:

https://www.youtube.com/watch?v=lPt-WQuniUs

Skip the stack part that is relevant for confocal stacks only and go
directly into the TrakEM2 section around 4:20.

Best,
Stephan


On Thu, 2021-01-07 at 20:56 +0000, Porter, George M.D. wrote:

> Hello,
>
> I am trying to stitch together montages of cardiac myocytes from
> electron micrographs and I find what I believe to be spherical
> aberration in the images (which apparently can happen with a side
> mount camera). Is there a way to correct this aberration in the
> images electronically using ImageJ or any other program? I have tried
> using the Warp Transformation and Lens Correction and Adaptive Wild
> Angle Filters in Photoshop without consistent success.
>
> Thanks,
> George Porter
>
> George A. Porter, Jr., MD, PhD
> Professor
> Chief and Fellowship Director, Division of Cardiology
> Department of Pediatrics
> University of Rochester Medical Center
> 601 Elmwood Ave.
> Box 631
> Rochester, NY  14642
> Phone: [phone]
> Fax: [phone]
> Email: [hidden email]
>
> "Let me say as I've always said, and I will always continue to say,
> that riots are socially destructive and self-defeating. ... But in
> the final analysis, a riot is the language of the unheard. And what
> is it that America has failed to hear? It has failed to hear that the
> plight of the Negro poor has worsened over the last few years. It has
> failed to hear that the promises of freedom and justice have not been
> met. And it has failed to hear that large segments of white society
> are more concerned about tranquility and the status quo than about
> justice, equality, and humanity. And so in a real sense our nation's
> summers of riots are caused by our nation's winters of delay. And as
> long as America postpones justice, we stand in the position of having
> these recurrences of violence and riots over and over again."
> Martin Luther King, Jr., "The Other America"
>
>
> --
> ImageJ mailing list:
> https://urldefense.com/v3/__http://imagej.nih.gov/ij/list.html__;!!Eh6p8Q!XlUDqXTkOrgmG0m032NSWI4MfXrikMIGrnrJV_KJz9dbJ3ERvC_qPGlxFYMVUffRyaiS$
>  

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html