Try the "extended depth of focus" plugin from BIG :
http://bigwww.epfl.ch/demo/edf/I tried it from time to time and the results are stunning.
Christophe
2006/10/17, NeLaS <
[hidden email]>:
> Hello,
>
> I have a series of light microscopy photos with different focal planes,
> taken under DIC. I was trying to create a focused composition image of this
> photos, but I'm not getting very good images.
>
> I'm creating a stack with the pictures and using the Stack Focuser plugin.
> If I use a small n (for the n x n kernel) like 3, I get the image in focus
> but with lots of blurring. If I use a bigger n (like 100) I get a better
> definition of the focused parts but the squares of the filter are very
> obvious, and some of them are not in focus at all!
>
> I know this images have a lot of unfocused noise which might be the
> responsible for the low quality result, but any suggestions in how to get
> focused stacks are very welcome!
>
> Is there any other tool/plugin I can use to do this?
>
> Thanks in advance!
>
> Bruno
>
> --
> Centro de Biologia Marinha (CEBIMar)
> Universidade de São Paulo
> Av. Manoel H. do Rego km 131,5
> 11600-000 São Sebastião, SP, Brasil
>
> www.myspace.com/nelas
>
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