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Stack Interval

Trina Sarkar
To Whom it May Concern:
Hello!
I am a research intern who is trying to count the number of cells in a
5,349-stack of images. However, the problem I continually run into is that
the "analyze particle" plugin I am using, is counting the same cells over
and over again because the cells have not yet left the image. Therefore, I
was wondering whether there is anyway to increase the time interval (in
seconds) between the individual stacks. Currently, the stacks are 0.03
seconds apart, but I would like to increase that. How can I do that?

Thank you so much,
Trina Sarkar

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Re: Stack Interval

Glen MacDonald-2
Dear Trina,
If you mean that the stack is a time-lapse series and you wish to sub-sample it, then consider the Image>Stack>Tools… to Reduce or Make Substack.  These can subsample the image series to increase the sampling interval.  

http://imagej.nih.gov/ij/docs/guide/146-28.html#toc-Subsection-28.6

Regards
Glen MacDonald
        Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
        Cellular Morphology Core
Center on Human Development and Disability
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[hidden email]







On Jul 17, 2013, at 1:04 PM, Trina Sarkar <[hidden email]> wrote:

> To Whom it May Concern:
> Hello!
> I am a research intern who is trying to count the number of cells in a
> 5,349-stack of images. However, the problem I continually run into is that
> the "analyze particle" plugin I am using, is counting the same cells over
> and over again because the cells have not yet left the image. Therefore, I
> was wondering whether there is anyway to increase the time interval (in
> seconds) between the individual stacks. Currently, the stacks are 0.03
> seconds apart, but I would like to increase that. How can I do that?
>
> Thank you so much,
> Trina Sarkar
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html