Stack equalization

[Knecht, David]

I have a time series stack of 16 bit phase contrast images.  They get increasingly darker with time because condensation gradually accumulated on the lid of the dish. Is there an easy way to generate an 8 bit stack in which the brightness is compensated during conversion so they all have about the same average brightness?  Thanks- Dave

Visiting Professor David Knecht
Beatson Institute for Cancer Research
University of Glasgow
Switchback Road, Bearsden
Glasgow Scotland G61 1BD
UK

[Aryeh Weiss]

On 4/16/12 3:55 PM, David Knecht wrote:

> I have a time series stack of 16 bit phase contrast images.  They get
> increasingly darker with time because condensation gradually
> accumulated on the lid of the dish. Is there an easy way to generate
> an 8 bit stack in which the brightness is compensated during
> conversion so they all have about the same average brightness?
> Thanks- Dave
>
> Visiting Professor David Knecht Beatson Institute for Cancer
> Research University of Glasgow Switchback Road, Bearsden Glasgow
> Scotland G61 1BD UK
>

Hi David,

You may be interested in the following post from teh list archive
https://list.nih.gov/cgi-bin/wa.exe?A2=ind1110&L=IMAGEJ&P=R13279&1=IMAGEJ&9=A&J=on&d=No+Match%3BMatch%3BMatches&z=4 

If that link does not work,  search for:
How to get uniform background on phase-contrast images

Regards
--aryeh
--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

[Knecht, David]

THanks for the suggestions.  I have not tried all, but I found a good solution in a Macro called "Bleach Correction" from Jens Rietdorf.  Dave

 http://www.embl.de/eamnet/html/body_bleach_correction.html 

On Apr 16, 2012, at 7:06 PM, Aryeh Weiss wrote:

> On 4/16/12 3:55 PM, David Knecht wrote:
>> I have a time series stack of 16 bit phase contrast images.  They get
>> increasingly darker with time because condensation gradually
>> accumulated on the lid of the dish. Is there an easy way to generate
>> an 8 bit stack in which the brightness is compensated during
>> conversion so they all have about the same average brightness?
>> Thanks- Dave
>>
>> Visiting Professor David Knecht Beatson Institute for Cancer
>> Research University of Glasgow Switchback Road, Bearsden Glasgow
>> Scotland G61 1BD UK
>>
>
> Hi David,
>
> You may be interested in the following post from teh list archive
> https://list.nih.gov/cgi-bin/wa.exe?A2=ind1110&L=IMAGEJ&P=R13279&1=IMAGEJ&9=A&J=on&d=No+Match%3BMatch%3BMatches&z=4 
>
> If that link does not work,  search for:
> How to get uniform background on phase-contrast images
>
> Regards
> --aryeh
> --
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph:  972-3-5317638
> FAX: [phone]
>

Visiting Professor David Knecht
Beatson Institute for Cancer Research
University of Glasgow
Switchback Road, Bearsden
Glasgow Scotland G61 1BD
UK