Well, I had the same experience and found the only solution to be measuring
without alignment. I believe it's the rotation especially that changes the
fluorescence.
I wrote a little plugin for that, basically a ROI manager to allow me to do
measurements on FRAP series. Don't know if it is what you need, but you can
take a look :
http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:frap_normalization:startIf the guidance pictures don't work, play a bit with the setting : putting
the alpha higher, generally connects more lines. Putting the tresholds
higher, gets rid of most of the noise.
Hope this helps
Kind regards
Joris
On Wed, Mar 11, 2009 at 8:23 PM, Tija Jacob <
[hidden email]> wrote:
> I have been using the image J stack reg plug in, and notice that after
> alignment, the fluorescence intensity of my planes has increased. Is there a
> way around this problem, as I need to do intensity measurements for time
> series experiments.
>
Locked
