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Steps to do DAB Quantifications

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hesham977

Steps to do DAB Quantifications

Dear All
I need your simple and accurate protocol for DAB Quantifications?

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*Dr. Hesham Noaman Abdelraheem Mustafa*
Assistant Professor, Faculty of Medicine, King Abdulaziz University,
ResearcherID <http://www.researcherid.com/rid/H-5618-2011>:
http://www.researcherid.com/rid/H-5618-2011
ORCID <http://orcid.org/0000-0003-1188-2187>:
http://orcid.org/0000-0003-1188-2187
Address: P.O.Box 80205 Jeddah, 21589 Saudi Arabia.
Phone: (Office): +966 2 6400000 Ext. 22016
(Mobile): +966 566 764 762
(Egypt): +20 1001 460 009
Official Email: [hidden email]

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Dr. Hesham N Mustafa Professor, Faculty of Medicine, King Abdulaziz University, ResearcherID: http://www.researcherid.com/rid/H-5618-2011 ORCID: http://orcid.org/0000-0003-1188-2187 Address: P.O.Box 80205 Jeddah, 21589 Saudi Arabia. Phone: (Office): +966 2 6400000 Ext. 22016 (Mobile): +966 566 764 762 (Egypt): +20 1001 460 009 Official Email: hmustafa@kau.edu.sa

sarahmmph

Re: Steps to do DAB Quantifications

Hi Dr. Hesham,

I asked the same question a month ago and i got the advice of using color deconvolution plugin followed by thresholding of brown image to determine area fraction of brown color. It is an easy method, however, in order to succeed the quality of images should be high, illumination well balanced & background should be neutral.

Hope this help and others participate to enrich the conversation.

Regards,

Sarah M. Mosaad, M.Sc.

School of Pharmacy,

Suez Canal University, Egypt.

On Thursday, March 5, 2015 7:55 PM, hesham977 [via ImageJ] <[hidden email]> wrote:

Dr. Hesham Noaman Abdelraheem Mustafa Assistant Professor, Faculty of Medicine, King Abdulaziz University, ResearcherID: http://www.researcherid.com/rid/H-5618-2011 ORCID: http://orcid.org/0000-0003-1188-2187 Address: P.O.Box 80205 Jeddah, 21589 Saudi Arabia. Phone: (Office): +966 2 6400000 Ext. 22016 (Mobile): +966 566 764 762 (Egypt): +20 1001 460 009 Official Email: [hidden email]

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