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Subtract Background Help

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Wilson R S (AT)

Subtract Background Help

Hi there,

I was wondering if there is a way of changing the default colour once
the subtract background function has been used.

At the moment it is set to black, and as I am looking at some dark
material it would be useful for me if the background was white not
black.

Thanking you for your help in advance.

Rhodri Wilson

Joachim Wesner

Antwort: Subtract Background Help

Hi Rhodri,

you can invert the whole image ("Invert LUT" or use other image processing)

IMHO only replacing one color out of 256 (or even up to 65536) in the LUT
is probably not what you want, if your background is not 100% flatr and
noisy, it will not work as you expect.

Maybe I do not understand what exactly "default color" means in your
case.....

Mit freundlichen Grüßen / Best regards

Joachim Wesner

"Wilson R S (AT)"
<[hidden email]
.UK> An
Gesendet von: [hidden email]
ImageJ Interest Kopie
Group
<[hidden email]. Thema
GOV> Subtract Background Help

27.02.2008 11:41

Bitte antworten
an
ImageJ Interest
Group
<[hidden email].
GOV>

Hi there,

I was wondering if there is a way of changing the default colour once
the subtract background function has been used.

At the moment it is set to black, and as I am looking at some dark
material it would be useful for me if the background was white not
black.

Thanking you for your help in advance.

Rhodri Wilson

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________

NeLaS

Re: Subtract Background Help

>
> I was wondering if there is a way of changing the default colour once

the subtract background function has been used.
>
> At the moment it is set to black, and as I am looking at some dark
> material it would be useful for me if the background was white not
> black.
>

Hey Rhodri,

Maybe I did not understand what you mean, but isn't a matter of ticking the
"light background" box in the subtract background dialog? Then you will get
a white background.

What version of ImageJ are you using?

cheers,

bruno

On Wed, Feb 27, 2008 at 7:59 AM, Joachim Wesner <
[hidden email]> wrote:

> Hi Rhodri,
>
> you can invert the whole image ("Invert LUT" or use other image
> processing)
>
> IMHO only replacing one color out of 256 (or even up to 65536) in the LUT
> is probably not what you want, if your background is not 100% flatr and
> noisy, it will not work as you expect.
>
> Maybe I do not understand what exactly "default color" means in your
> case.....
>
> Mit freundlichen Grüßen / Best regards
>
> Joachim Wesner
>
>
>
>
> "Wilson R S (AT)"
> <[hidden email]
> .UK> An
> Gesendet von: [hidden email]
> ImageJ Interest Kopie
> Group
> <[hidden email]. Thema
> GOV> Subtract Background Help
>
>
> 27.02.2008 11:41
>
>
> Bitte antworten
> an
> ImageJ Interest
> Group
> <[hidden email].
> GOV>
>
>
>
>
>
>
> Hi there,
>
>
>
> I was wondering if there is a way of changing the default colour once
> the subtract background function has been used.
>
> At the moment it is set to black, and as I am looking at some dark
> material it would be useful for me if the background was white not
> black.
>
>
>
> Thanking you for your help in advance.
>
>
>
> Rhodri Wilson
>
>
>
> ______________________________________________________________________
> This email has been scanned by the MessageLabs Email Security System.
> For more information please visit http://www.messagelabs.com/email
> ______________________________________________________________________
>

--
Bruno C. Vellutini
organelas.com | ccnelas.org | desertoresdaescada.com

Centro de Biologia Marinha (CEBIMar)
Universidade de São Paulo
Av. Manoel H. do Rego km 131,5
11600-000 São Sebastião, SP, Brasil
http://www.usp.br/cbm/

Wilson R S (AT)

Re: Subtract Background Help

Thanks for all the help have decided to use a, sub image instead to get rid of background noise.

Rhodri
-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of NeLaS
Sent: 27 February 2008 12:34
To: [hidden email]
Subject: Re: Subtract Background Help

>
> I was wondering if there is a way of changing the default colour once

the subtract background function has been used.
>
> At the moment it is set to black, and as I am looking at some dark
> material it would be useful for me if the background was white not
> black.
>

Hey Rhodri,

Maybe I did not understand what you mean, but isn't a matter of ticking the
"light background" box in the subtract background dialog? Then you will get
a white background.

What version of ImageJ are you using?

cheers,

bruno

On Wed, Feb 27, 2008 at 7:59 AM, Joachim Wesner <
[hidden email]> wrote: