Subtract Background and stacks

[Julin Maloof]

Hello,

I get a very different results when I use the Subtract Background command on an image compared to using it on an image that is part of a stack.  When processing a single image the command behaves as I would expect and I get a very nice result.  When using it on a stack (or even on a single image in a stack), it appears that the image is thresholded...what should be background goes to black (even though the "white background" box is checked) and the rest of the image goes to white.  Is this a bug, or am I doing something wrong, or is this the expected behavior?

Thanks,

Julin


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Julin Maloof
Assistant Professor
Section of Plant Biology
University of California, Davis
1 Shields Ave
Davis, CA, 95616

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