|
|
Hello Listers,
I would like to thank everybody for trying to help with the awful axon image and how to count them. I found out that the person did not coverslip the sections, helped make the images look bad. Plus the stitching is awful, she didn't use on of my microscopes! ;)
She is trying to separate the axons by area size, so I was mistaken on that point.
Her problem is that with the uniform staining (everything is gray/blue) that the axons appear to touch each other when she thresholds the axons and she doesn't want to spend a lot of time manually separating the touching axons.
I think she's going to have to do a lot of steps manually, that stinks but that's science.
I'm going to help her to get better images and see what happens.
So, once again, thank you for all your assistance.
Paula :-)
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
|
Locked
