Hi
I would like to ask about the suitable choice for threshold method to quantify the area fraction of brown color.
I have H/DAB images for treated groups. I need to use a method that is least subjective, gives consistent results meaning if i use it several times gives the exact same area fraction.
There are several methods:
1, Image>>adjust>>threshold, moving brightness and hue sliders to get 2 areas then divide them to get area fraction.
2. Image>>color>>split. choose green chanel. Image>>adjust>>threshold>>clt+M
3. www.med.upenn.edu/cellbio/documents/imagej_colorsegmentpdf.pdf
Image.. Plugins..threshold color...move saturation slider to select ROI..close plugin..image..type...8bits..image..adjust...t hreshold..clt+M.
I know there may be other methods for color segmentation but thats all i know. I cant use color deconvolution as i cant do background correction calculation ...i am just a pharmacologist.
Thanks in advance.
Sarah M.Mosaad, M.Sc.
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html
Locked
