> I am having difficulty creating binary images using the
> Threshold that is called from Image-Adjust. I want to
> generate a binary where the feature of interest is gray 255
> and the remainder is 0. I used the picker to set the gray
> value to 255 and use the scroll bars to set upper and lower
> bounds. Interestingly, I still see low count noise even
> after the lower bound is set well above the noise gray
> levels. Next, when attempting to create the binary, a
> window detailing options "Apply LUT' appears where I
> de-select the option for black foreground, white
> background. The resulting window is a uniform level (62 in
> todays attempt) using the red option; using the black and
> white option in another attempt results in a uniformly gray
> image, again at level 62. Another attempt using the
> Black-White option and picker set to 255 resulted in the
> entire image being set to 255. I am using ImageJ 1.34S on
> a G5.
The easiest way to do this is to check "Black foreground, white
background" in the "Apply LUT" dialog and then press shift-i
(Edit>Invert).
-wayne
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