Trouble with image measurements

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Trouble with image measurements

Jfallica13
I am currently working on measuring various length in histological sections.  I have a grid overlay that measures about 20,000+ line lengths in each section.  The discrepancy is that when I open the image in Photoshop to edit and then transfer it to imagej all of the line lengths increase in length by about 1 micrometer in comparison to just opening the tif in imagej.  Is there a difference in the way a tif is saved in photoshop that would change the picture size or pixel ratio?  I  am really interested to find out how this could be resolved.  
Thanks.  

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Re: Trouble with image measurements

Michael Schmid
Hi,

sorry your question does not rally make the problem clear,
I fear.

> all of the line lengths increase in length by about 1 micrometer
?? what does that mean??
What is your image size, and how many pixels correspond to 1 um?

If I take an image with spatial calibration, open and save it
in Photoshop, the spatial calibration is gone. So, in ImageJ
it won't give distances in micrometers, only in pixels.

Michael
________________________________________________________________

On 8 May 2007, at 17:28, Jfallica13 wrote:

> I am currently working on measuring various length in histological  
> sections.
> I have a grid overlay that measures about 20,000+ line lengths in each
> section.  The discrepancy is that when I open the image in  
> Photoshop to edit
> and then transfer it to imagej all of the line lengths increase in  
> length by
> about 1 micrometer in comparison to just opening the tif in  
> imagej.  Is
> there a difference in the way a tif is saved in photoshop that  
> would change
> the picture size or pixel ratio?  I  am really interested to find  
> out how
> this could be resolved.
> Thanks.
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Angle tool not coming up

B. Prabhakar Pandian
Hello,
        I am using ImageJ Version 1.37 and I see that there is no angle
measurement tool in the measurement settings. Has it been removed
or its in another location.

Thanks,

Prabhakar
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Re: Trouble with image measurements

Jfallica13
In reply to this post by Michael Schmid
Hello,

I actuallu finally figured it out and it did have something to do with the calibration settings and how the numbers generated were being rounded.

Thanks for the input,

Jon

Michael Schmid-3 wrote
Hi,

sorry your question does not rally make the problem clear,
I fear.

> all of the line lengths increase in length by about 1 micrometer
?? what does that mean??
What is your image size, and how many pixels correspond to 1 um?

If I take an image with spatial calibration, open and save it
in Photoshop, the spatial calibration is gone. So, in ImageJ
it won't give distances in micrometers, only in pixels.

Michael
________________________________________________________________

On 8 May 2007, at 17:28, Jfallica13 wrote:

> I am currently working on measuring various length in histological  
> sections.
> I have a grid overlay that measures about 20,000+ line lengths in each
> section.  The discrepancy is that when I open the image in  
> Photoshop to edit
> and then transfer it to imagej all of the line lengths increase in  
> length by
> about 1 micrometer in comparison to just opening the tif in  
> imagej.  Is
> there a difference in the way a tif is saved in photoshop that  
> would change
> the picture size or pixel ratio?  I  am really interested to find  
> out how
> this could be resolved.
> Thanks.
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Re: Trouble with image measurements

Jfallica13
In reply to this post by Michael Schmid
Hello,

I actuallu finally figured it out and it did have something to do with the calibration settings and how the numbers generated were being rounded.

Thanks for the input,

Jon

Michael Schmid-3 wrote
Hi,

sorry your question does not rally make the problem clear,
I fear.

> all of the line lengths increase in length by about 1 micrometer
?? what does that mean??
What is your image size, and how many pixels correspond to 1 um?

If I take an image with spatial calibration, open and save it
in Photoshop, the spatial calibration is gone. So, in ImageJ
it won't give distances in micrometers, only in pixels.

Michael
________________________________________________________________

On 8 May 2007, at 17:28, Jfallica13 wrote:

> I am currently working on measuring various length in histological  
> sections.
> I have a grid overlay that measures about 20,000+ line lengths in each
> section.  The discrepancy is that when I open the image in  
> Photoshop to edit
> and then transfer it to imagej all of the line lengths increase in  
> length by
> about 1 micrometer in comparison to just opening the tif in  
> imagej.  Is
> there a difference in the way a tif is saved in photoshop that  
> would change
> the picture size or pixel ratio?  I  am really interested to find  
> out how
> this could be resolved.
> Thanks.
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Re: Trouble with image measurements

Jfallica13
In reply to this post by Michael Schmid
Hello,

I actually finally figured it out and it did have something to do with the calibration settings and how the numbers generated were being rounded.

Thanks for the input,

Jon

Michael Schmid-3 wrote
Hi,

sorry your question does not rally make the problem clear,
I fear.

> all of the line lengths increase in length by about 1 micrometer
?? what does that mean??
What is your image size, and how many pixels correspond to 1 um?

If I take an image with spatial calibration, open and save it
in Photoshop, the spatial calibration is gone. So, in ImageJ
it won't give distances in micrometers, only in pixels.

Michael
________________________________________________________________

On 8 May 2007, at 17:28, Jfallica13 wrote:

> I am currently working on measuring various length in histological  
> sections.
> I have a grid overlay that measures about 20,000+ line lengths in each
> section.  The discrepancy is that when I open the image in  
> Photoshop to edit
> and then transfer it to imagej all of the line lengths increase in  
> length by
> about 1 micrometer in comparison to just opening the tif in  
> imagej.  Is
> there a difference in the way a tif is saved in photoshop that  
> would change
> the picture size or pixel ratio?  I  am really interested to find  
> out how
> this could be resolved.
> Thanks.