Turboreg: Problem with jpg and png image format

[JoLi]

Hello everybody,

I have a problem I can't figure out alone any more. Out of my microscope data I create image files using mathlab. with turboreg I want to align the images with respect to another. This works perfekt when I create .jpg files with mathlab, but if I use .png or even .tif images the error "At least two grayscale or RGB-stack images are required".
I tried to get rid of this problem changing the export options in mathlab to grayscale, but it doesn't work. This option is also under the "rendering" dialog box, so i am even not sure if this is the right place to change the export options but I didn't finde any other options.

I want to use .png images because it is a lossles compression and .jpg smoothes my data, though changing it a bit which is not wanted. My data is only in grayscale, but the images seem to appear as colour, what I don't understand. I would be very happy if anybody has some ideas.

Thank you.

Jonas

[Joel Sheffield]

Hi Jonas,

Maybe, even though you convert to grayscale using mathlab, the program
saves the file in an rgb format.  You can convert to an 8-bit grayscale
image in ImageJ by loading the images, and selecting Image>type, and
selecting 8-bit grayscale as an option.

Joel


On Wed, Mar 6, 2013 at 12:56 PM, JoLi <[hidden email]>wrote:

> Hello everybody,
>
> I have a problem I can't figure out alone any more. Out of my microscope
> data I create image files using mathlab. with turboreg I want to align the
> images with respect to another. This works perfekt when I create .jpg files
> with mathlab, but if I use .png or even .tif images the error "At least two
> grayscale or RGB-stack images are required".
> I tried to get rid of this problem changing the export options in mathlab
> to
> grayscale, but it doesn't work. This option is also under the "rendering"
> dialog box, so i am even not sure if this is the right place to change the
> export options but I didn't finde any other options.
>
> I want to use .png images because it is a lossles compression and .jpg
> smoothes my data, though changing it a bit which is not wanted. My data is
> only in grayscale, but the images seem to appear as colour, what I don't
> understand. I would be very happy if anybody has some ideas.
>
> Thank you.
>
> Jonas
>
>
>
>
> --
> View this message in context:
> http://imagej.1557.n6.nabble.com/Turboreg-Problem-with-jpg-and-png-image-format-tp5002040.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

... [show rest of quote]

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[JoLi]

Hey Joel,

yeah, that was the problem. I will have to work at the matlab output, the pictures are still truecolor, which means rgb. With the change in format via imagej the problem is solved.

Thank you !