Two channel color merge

[Daniel James White]

Hi Heera

it would be good for you to look through the archives of this email list
to answer your question, before you ask a very often asked question,
as there is much useful information already written by me and others.

Basically, see
http://fiji.sc/Colocalization_Analysis
http://fiji.sc/Colocalization_-_hardware_setup_and_image_acquisition

cheers

Dan


On Oct 31, 2011, at 5:01 AM, IMAGEJ automatic digest system wrote:

> There is 1 message totaling 43 lines in this issue.
>
> Topics of the day:
>
>  1. Two channel color merge
>
> ----------------------------------------------------------------------
>
> Date:    Sun, 30 Oct 2011 19:04:20 -0700
> From:    Diamond <[hidden email]>
> Subject: Re: Two channel color merge
>
> I am jumping on the thread a tad late, but i have similar problems and hope
> to get some help. I have two channels in whose colocalization i am
> interested in. Both the channel are of different intensities and and hence i
> am having a trouble getting a "yellow" merge image.
>
> From literature available, i understand that the following procedure may
> serve better to show colocalization in my case. I am not sure whether i have
> understood it right and i would appreciate your feedback.
>
> 1) Apply Magenta LUT to my red channel.
> 2) Apply Cyan LUT to my green channel.
> 3) Covert the images to RGB and perform a difference operation using Image
> Calculator or Use color function -color merge using the difference operator.
> 4) In the resulting image: Yellow reflects colocalization where the pixels
> are of equal intensity.
>                                    Green reflects colocalization with cyan
> being of brighter intensity than magenta.
>                                    Red reflects colocalization with magenta
> being of brighter intensity than cyan.
>
> I have performed quantification on my images and it does suggest
> colocalization. Is the above mentioned method correct if i want to publish
> my images ?
>
> Would really really appreciate your response !
> Thanks a lot,
> Cheers
> Heera

Dr. Daniel James White BSc. (Hons.) PhD

Leader - Image Processing Facility,
Senior Microscopist,
Light Microscopy Facility.

Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

[phone] (German Mobile)
[phone] (Work phone at MPI-CBG)
[phone] (Fax MPI-CBG LMF)
chalkie666 Skype
http://www.bioimagexd.net  BioImageXD
http://fiji.sc                                        Fiji -  is just ImageJ (Batteries Included)
http://www.chalkie.org.uk                Dan's Homepages
https://ifn.mpi-cbg.de  Biopolis Dresden Imaging Platform (BioDIP)
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )