Dear community,
Please excuse me as this is not exactly a solely ImageJ related question,
but ImageJ List is the biggest and most diverse community of specialists in
digital image processing and analysis that I have access to (and also the
most reliable). So to the point:
I turn to all of you that have experience and expertise in the stereology
methods. As stereology requires UIR sampling and UIR sections are something
that any specialist in the life sciences would avoid, it is only natural
that we would turn to UIR volume probes instead. My question is about the
spherical volume probe. In its classical implementation the spherical
volume is used to probe a series of optical or physical z-sections. This
approach is however somewhat limited as in reality it can only be
implemented on confocal or high magnification wide field epifluorescence
images, which requires expensive and time consuming preparation and image
acquisition not to mention that the sampled volume is relatively small. It
is almost unusable for bright field or low magnification epifluorescence
images (as for the time being there exist no good deconvolution techniques
for them).
So I asked my self and I couldn’t answer on my own reliably (as I am not
very experienced in mathematics and stereology, and being unable to
implement the latter in UIR way I stayed away from it in my research
(although I experimented with it now and than)), the following question:
Is it possible to turn around the spherical volume around in the
following manner?:
Instead of probing a series of z-sections with one spherical volume,
isn't it equally valid to probe series of single sections (or even maximum
intensity projections of thick sections) with several randomly placed
intersections of the given volume, i.e. to probe the sections with several
circles of different size where the diameter of each of the circles is
equal to the diameter of the sectional profile of the spherical volume at
1/n of its height where n is a random integer?
Best regards
Stoyan Pavlov
---
Dr. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
Bulgaria
Tel: +359 (0) 52 - 677 - 052
e-mail:
[hidden email]
[hidden email]
Zentrum für Anatomie
Uniklinik Köln
[hidden email]
Tel: +49221/478-5730
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