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Recently, I have found that when I try to select an ROI, the color channel that ROI was created in is automatically selected as well. Moreover, when I create my ROIs with a macro, "remove slice info" has no effect on them. In the past, I have always been able to generate ROIs devoid of slice and channel information. Here is a sample of the code I've been using:
getCursorLoc(x, y, z, flags);
makeOval(x-radius, y-radius, radius*2, radius*2);
roiManager("Add");
I also have a few lines that manipulate the name of the ROI, but I've omitted this for concision.
Any assistance anyone is able to render would be greatly appreciated.
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