Urgent: how to do morphometric analysis??

[Chen, Yuk Shan]

Dear all,
     Currently I am trying to use the Image J (1.37v) to do the morphometric
analysis of the toluidin-blue stained nerve sections: e.g. myelinated fiber
size, fascicular area, circularity, distribution...I have been trying very hard
to explore the program, but still find it very difficult. Actually before for
large or single objects, I did not have problems to use Image I to measure the
area or circularity. However, now I find it difficult because the fiber area is
small and, in addition, the  number of fibers is great. Can anyone help???


Thank you very very much!!!

YuShan Chen

[Chengxin Li]

I am not sure if I understand your problem. But you may have a try to convert the image to 8bit, threshold, particle analysis, ...

Good luck !

Chengxin

>>> "Chen, Yuk Shan" <[hidden email]> 04/18/08 4:40 AM >>>
Dear all,
     Currently I am trying to use the Image J (1.37v) to do the morphometric
analysis of the toluidin-blue stained nerve sections: e.g. myelinated fiber
size, fascicular area, circularity, distribution...I have been trying very hard
to explore the program, but still find it very difficult. Actually before for
large or single objects, I did not have problems to use Image I to measure the
area or circularity. However, now I find it difficult because the fiber area is
small and, in addition, the  number of fibers is great. Can anyone help???


Thank you very very much!!!

YuShan Chen