Eric,
One way to create a Variance image from a stack would be as follows: With
the stack open in ImageJ, go to Image > Stacks > Z Projection. In the Z
Projection window select standard deviation and click OK. Then Square the
standard deviation image by going to Process > Math > Square. If you need
to do this many times, you can record a macro of the process. To record a
macro go to Plugins > Macros > Record and then run through the process of
creating the variance image. I hope that helps.
Cheers,
Aaron.
On Wed, Dec 19, 2012 at 5:20 AM, Eric Barnhill <
[hidden email]>wrote:
> Given a stack, I would like to create a summary image of the variance of
> each pixel in the stack as it goes down the Z axis, that is to say, the
> variance point to point in the "plot Z axis profile" function.
>
> A simpler way to describe it is, I want to capture how much each point is
> flickering as opposed to changing smoothly.
>
> Has anyone seen a plugin that does something like this, and if not, would
> there be a way to access this information through the "ZAxisProfiler"
> object?
>
> Thanks,
> Eric
>
> ---
> Eric Barnhill
> Clinical Research Imaging Centre
> The University of Edinburgh
>
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