View images with 12 channels

[Pang, Zhengyu (GE Global Research)]

Dear all,

I have a set of images obtained using 12 different channels and I want
to view them. They were all obtained using monochrome camera, therefore
I got a tif file with 12 channels. When I tried to view some of these
channels for the correlation between these channels, I found that I
could not use composite mode, but only use color mode which allows me to
view only one channel at a time. In addition, I found that the "Channel
Tool" have a maximum of 7 channels, and no way for me to select/deselect
channels from 8-12. Do I have to use macro to view it?

Is there any way for me to get around of them? And let me to view 3
channels at a time.

Thanks!

                        Zhengyug Global Research Center
>
> ______________________________________________________________________
>
>           Zhengyu Pang, Ph.D.
                        Bioanalytics and Protein Science Laboratory
                        Biosciences Global Technology Organization
> One Research Circle, K1 5B37A
> Niskayuna, NY12309
> * [hidden email]
> *[phone]
>
>
>
>

[Stephan Saalfeld]

Dear Zhengyu Pang,

did you try Image5D already?

http://rsb.info.nih.gov/ij/plugins/image5d.html

To my best knowledge, ImageJ itself so far neglects the existence of
wavelength as a continuous dimension that allows imaging data with any
number of channels and focuses on the human perceptible rgb.

Best regards,
Stephan


On Wed, 2008-12-17 at 11:54 -0500, Pang, Zhengyu (GE, Research) wrote:

> Dear all,
>
> I have a set of images obtained using 12 different channels and I want
> to view them. They were all obtained using monochrome camera,
> therefore
> I got a tif file with 12 channels. When I tried to view some of these
> channels for the correlation between these channels, I found that I
> could not use composite mode, but only use color mode which allows me
> to
> view only one channel at a time. In addition, I found that the
> "Channel
> Tool" have a maximum of 7 channels, and no way for me to
> select/deselect
> channels from 8-12. Do I have to use macro to view it?
>
> Is there any way for me to get around of them? And let me to view 3
> channels at a time.
>
> Thanks!
>
> Zhengyug Global Research Center
> >
> >
> ______________________________________________________________________
> >
> >           Zhengyu Pang, Ph.D.
> Bioanalytics and Protein Science Laboratory
> Biosciences Global Technology Organization
> > One Research Circle, K1 5B37A
> > Niskayuna, NY12309
> > * [hidden email]
> > *[phone]
> >
> >
> >
> >

[Pang, Zhengyu (GE Global Research)]

Stephen,

I downloaded image5D. Yes, it can view the images in 12 channels.
However, it did not allow me to assign the color for each channel. All
the color is pre-determned.

Anyway, thank for your help. At least it gives me a way to see my
images.

Zhengyu

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Stephan Saalfeld
Sent: Thursday, December 18, 2008 9:29 AM
To: [hidden email]
Subject: Re: View images with 12 channels

Dear Zhengyu Pang,

did you try Image5D already?

http://rsb.info.nih.gov/ij/plugins/image5d.html

To my best knowledge, ImageJ itself so far neglects the existence of
wavelength as a continuous dimension that allows imaging data with any
number of channels and focuses on the human perceptible rgb.

Best regards,
Stephan


On Wed, 2008-12-17 at 11:54 -0500, Pang, Zhengyu (GE, Research) wrote:
> Dear all,
>
> I have a set of images obtained using 12 different channels and I want

> to view them. They were all obtained using monochrome camera,
> therefore I got a tif file with 12 channels. When I tried to view some

> of these channels for the correlation between these channels, I found
> that I could not use composite mode, but only use color mode which
> allows me to view only one channel at a time. In addition, I found
> that the "Channel Tool" have a maximum of 7 channels, and no way for
> me to select/deselect channels from 8-12. Do I have to use macro to
> view it?
>
> Is there any way for me to get around of them? And let me to view 3
> channels at a time.
>
> Thanks!
>
> Zhengyug Global Research Center
> >
> >
> ______________________________________________________________________
> >
> >           Zhengyu Pang, Ph.D.
> Bioanalytics and Protein Science Laboratory
> Biosciences Global Technology Organization
> > One Research Circle, K1 5B37A
> > Niskayuna, NY12309
> > * [hidden email]
> > *[phone]
> >
> >
> >
> >