Viewing RGB stacks: Can you toggle channel visibility

[Michael Murray-5]

Hi,
   Is there any way of independently toggling the visibility of the  
red, green and blue channels of an RGB image stack.

   A related issue is how to change the brightness/contrast/gamma of  
individual channels without actually modifying the underlying pixel  
values (i.e. I sort of want to have LUT control of channels on top of  
a non-LUT image).

Apologies if this has been dealt with before - I could find nothing  
in the archives.

thanks in advance,
Mike Murray





Dr Michael J. Murray
Postdoctoral Fellow,
Centre for the Molecular Genetics of Development,
Research School of Biological Sciences,
Building 46, The Australian National University,
Canberra City ACT 2601,
Australia

Phone: [phone]
Fax:      [phone]

[Joachim Walter]

Try Image5D
http://rsb.info.nih.gov/ij/plugins/image5d.html
After you put the jar-file into the plugins-directory you get sub-menu
Plugins->Image5D.

For your application there is a plugin "RGB to Image5D". This converts
your RGB-Stack to an Image5D with three channels and a slider to select
the displayed channel.

To view all channels at the same time, select "ovl" in the dropdown-box
above the slider. This replaces the slider by a control with a field for
each channel. Each field contains a square checkbox that controls, if
the channel is displayed or not.

You can control brightness and contrast of each channel individually
with the Adjust->Brightness/Contrast dialog of ImageJ. To select, which
channel the dialog acts on, use the round radiobuttons in the fields.
When you click on the "Color" button, you get a control to edit the LUT
of a channel.

You can save and open Image5Ds with the respective plugins, so you don't
have to use "RGB to Image5D" every time you want to view an image like
this and you also save the display settings you chose.
All this also works with more or less than three channels. Just you
cannot use "RGB to Image5D" to make an Image5D from your data. The
plugins "Open Series as Image5D" and "Stack to Image5D" are meant for
this case.

All the best,

Joachim




Michael Murray schrieb:

> Hi,
>   Is there any way of independently toggling the visibility of the  red,
> green and blue channels of an RGB image stack.
>
>   A related issue is how to change the brightness/contrast/gamma of  
> individual channels without actually modifying the underlying pixel  
> values (i.e. I sort of want to have LUT control of channels on top of  a
> non-LUT image).
>
> Apologies if this has been dealt with before - I could find nothing  in
> the archives.
>
> thanks in advance,
> Mike Murray
>
>
>
>
>
> Dr Michael J. Murray
> Postdoctoral Fellow,
> Centre for the Molecular Genetics of Development,
> Research School of Biological Sciences,
> Building 46, The Australian National University,
> Canberra City ACT 2601,
> Australia
>
> Phone: [phone]
> Fax:      [phone]

--
-----------------------------------------------
Dr. Joachim Walter

TILL I.D. GmbH
c\o BioImaging Zentrum
Großhaderner Str. 2
D-82152 Martinsried

Tel.: [phone]
Fax:  [phone]

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