Volume calculation

[Manuel Palacios-2]

Dear All,

I have a Z stack of confocal images from a film which is coated in a  
microwell. The film is aprox. 15 microns thick., but it is not  
perfectly homogeneous through all the surface. I need to measure the  
total volumen of the semi-spheric film. Is there anyway to do it?...  
So far what I have checked in the web works for solids (like cells)  
but I can not a find a way to do just the semi-spheric film.

Thanks and best regards

Manuel

---------------------------------------------------------------
Lic. Manuel Alfredo Palacios H.
Doctoral Fellow
McMaster Outstanding Research Fellow of Distinction
[hidden email]


Center for Photochemical Sciences
Bowling Green State University
Bowling Green, OH, 43403
Phone: [phone] (Lab)
http://personal.bgsu.edu/~manuelp


"In theory, there's no difference between theory and practice, but in  
practice there is"
Jan Van Snepscheut
---------------------------------------------------------------

[Robert Dougherty]

Manuel,

It may be a job for Measure Stack:  http://www.optinav.com/MeasureStack.htm.


Bob

Robert P. Dougherty, Ph.D.
President, OptiNav, Inc.
Phone [phone]
Fax [phone]
www.optinav.com
 

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Manuel Palacios
> Sent: Thursday, September 13, 2007 5:01 PM
> To: [hidden email]
> Subject: Volume calculation
>
> Dear All,
>
> I have a Z stack of confocal images from a film which is coated in a
> microwell. The film is aprox. 15 microns thick., but it is not
> perfectly homogeneous through all the surface. I need to measure the
> total volumen of the semi-spheric film. Is there anyway to do it?...
> So far what I have checked in the web works for solids (like cells)
> but I can not a find a way to do just the semi-spheric film.
>
> Thanks and best regards
>
> Manuel
>