Volume of water ingression

[Rainer Wechselberger]

Hi,
I'm a newbie. I spend a couple of hours on tutorials and in the manual but couldn't find how to do this. Maybe one of the experienced users could help me? It's a simple task (or so I thought): I've got jpeg images of water ingression into tablets from a bench top imaging system. I would like to make a graph which documents this process over time. I've got a simple software on the instrument which can do this. Define a point in the image, define "ascenting direction" - and it will draw a contour line around the (dark) core of the tablet (the wet outside is in grey shades) and give me the area within this contour line. I can even append this value to a csv file. The problem: There is no batch mode and I've got more than a 1000 images... That's when I installed ImageJ. Unfortunately I got stuck here. Won't find how to do the contour thing and get the area inside. Also woudn't know how to do it in batch mode and get a txt file with the results for the whole series.
Obviously this is not about complicated anatomical structures... Can anybody help me nevertheless?

Thanks a lot!

Rainer

[Cammer, Michael]

Maybe a simple loop of opening an image, autothresholding, measuring the area change over time, and repeating and then pooling the results would do this.  Could you upload an example image to a file sharing service?  I bet that this is easy to solve and somebody on this list could help you.
Regards,
Michael
________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Rainer Wechselberger
Sent: Monday, April 02, 2012 11:49 AM
To: [hidden email]
Subject: Volume of water ingression

Hi,
I'm a newbie. I spend a couple of hours on tutorials and in the manual but couldn't find how to do this. Maybe one of the experienced users could help me? It's a simple task (or so I thought): I've got jpeg images of water ingression into tablets from a bench top imaging system. I would like to make a graph which documents this process over time. I've got a simple software on the instrument which can do this. Define a point in the image, define "ascenting direction" - and it will draw a contour line around the (dark) core of the tablet (the wet outside is in grey shades) and give me the area within this contour line. I can even append this value to a csv file. The problem: There is no batch mode and I've got more than a 1000 images... That's when I installed ImageJ. Unfortunately I got stuck here. Won't find how to do the contour thing and get the area inside. Also woudn't know how to do it in batch mode and get a txt file with the results for the whole series.
Obviously this is not about complicated anatomical structures... Can anybody help me nevertheless?

Thanks a lot!

Rainer