alternative to doWand(x,y)

[chepe santacruz]

I am trying to analyze images of lung samples and in order to get the
measurements of a particular alveolar i use the imageJ "doWand(x,y)"
method to select it(the image is converted to a black and white version
beforehand) then i get it's roi. In order to do this i have to open the
image and show it on the
screen.
Is there a way to select a region without opening the image on the screen? I
tried to use the ImageProcessor and manipulate it's pixel array but there is
no way to select a region.

Would anyone have a solution?

-----------------------------------------
Billy Bustillo
McGill University Meakins Laboratories
Montreal, Canada

[Albert Cardona]

Chepe,

There is no need to have the images visible in the screen to do whatever on
them. In this case, you can open the image from a plugin and execute the
doWand(x,y) on it without ever showing the image:

// open and set active
ImagePlus imp = new Opener().openImage("/path/to/image.tif");
if (null == imp) return;
WindowManager.setTempCurrentImage(imp);

// analyze
IJ.doWand(x, y);
IJ.run("Measure");


// If you would like to put in any other kind of ROI, simply create it (it's
an object) and add it to the ImagePlus:

int[] xpoints = new int[]{0, 100, 100, 0};
int[] ypoints = new int[]{0, 0, 100, 100};
Roi roi = new PolygonRoi(xpoints, ypoints, xpoints.length, Roi.POLYGON);
imp.setRoi(roi);
IJ.run("Measure");

// And don't forget to cleanup after being finished with the image!

// cleanup
imp.flush();
WindowManager.setTempCurrentImage(null);



Albert

--
Albert Cardona
Molecular Cell Developmental Biology
University of California Los Angeles
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