analyse particles

[Min=?UTF-8?Q?=C3=83=C2=A9_?=Zantow]

Dear All

I am working are a project to determine the size and shape of particles. I
would like to use the analyse particle feature. I have subtracted the
background and thresholded the image.

Could anyone please assist me to process this image so that all the
particles can be detected with the analyse particle feature. I would also
like to be able to split the particle using the watershed algorithm.

I have attached the original image.

Any help would be much appreciated.

Regards

Miné

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[David Webster]

Mine,

If you objects are always appr. circular, you could try using the circular
Hough Transform. I have a version of the one from the ImageJ plugins page
that will put estimated locations and radii in a Results Table.

I am enclosing (I hope) and example, make by first running Process/Make
Binary, then Process/Binary/Make Binary. Make sure the edges/contour are
the 255 values.
Then run the HT. The results are shown as a red overlay.

If you are interested, I will zip up my software and send it to you.

David Webster




[image: Inline image 1]

On Wed, Jun 20, 2012 at 5:07 AM, Miné Zantow <[hidden email]> wrote:

> Dear All
>
> I am working are a project to determine the size and shape of particles. I
> would like to use the analyse particle feature. I have subtracted the
> background and thresholded the image.
>
> Could anyone please assist me to process this image so that all the
> particles can be detected with the analyse particle feature. I would also
> like to be able to split the particle using the watershed algorithm.
>
> I have attached the original image.
>
> Any help would be much appreciated.
>
> Regards
>
> Miné
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html