You could manually select and measure the area of several of the
particles. If you do not set a scale, then the area will be in
pixel^2. Then you can use the range of measurements as your limits.
Justin
On 8/30/07, chaofang yue <
[hidden email]> wrote:
> Dear group:
> I am trying to use the Analyze--> analyze particles to count the number of
> particles that are on a phase contrast microscopic image. I know roughly my
> particle size, but it seems to me ImageJ requires a size range expressed in
> "pixel^2". How could I relate the actual size of the particles to the
> pixels. Thank you very much for any suggestions! Chaofang
>
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