openfile="d:\\imagej\\demo2.raw";
run("Raw...", "open=&openfile image=[16-bit Signed] width=1280 height=960 offset=0 number=1 gap=0 little-endian");
run("Subtract...", "value=400");
run("Macro...", "code=v=log(v)*255/log(4096)");
makeRectangle(400, 224, 298, 174);
run("Measure");

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