average thickness of an irregular-shaped object measurement

[Soriano.Joaquim]

Hi everyone!
Does anyone know how to measure the average thickness of an irregular-shaped object such as the one in the attachment?
Thanks in advance!

Joaquim Soriano Felipe.
Confocal Microscopy Unit
Spanish National Cancer Research Centre (CNIO)
Melchor Fernández Almagro,3, E-28029 Madrid, Spain
Phone  + 34 917 328 000, exts.: 3161, 3162. [hidden email]<mailto:[hidden email]>



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[vbindokas]

Hi Joaquim,
You can use the "local thickness" plugin to fill the white space with
respective thicknesses, then threshold all of it and measure the mean
thickness. The plugin is built into FIJI, or download from:
http://www.optinav.com/imagej.html
The stripe has a mean of 32.14 pixels. See the plugin documentation for
details.
cheers,


On 6/17/2014 10:45 AM, Soriano.Joaquim wrote:

> Hi everyone!
> Does anyone know how to measure the average thickness of an irregular-shaped object such as the one in the attachment?
> Thanks in advance!
>
> Joaquim Soriano Felipe.
> Confocal Microscopy Unit
> Spanish National Cancer Research Centre (CNIO)
> Melchor Fernández Almagro,3, E-28029 Madrid, Spain
> Phone  + 34 917 328 000, exts.: 3161, 3162. [hidden email]<mailto:[hidden email]>
>
>
>
> **NOTA DE CONFIDENCIALIDAD** Este correo electrÿnico, y en su caso los ficheros adjuntos, pueden contener informaciÿn protegida para el uso exclusivo de su destinatario. Se prohÿbe la distribuciÿn, reproducciÿn o cualquier otro tipo de transmisiÿn por parte de otra persona que no sea el destinatario. Si usted recibe por error este correo, se ruega comunicarlo al remitente y borrar el mensaje recibido.
> **CONFIDENTIALITY NOTICE** This email communication and any attachments may contain confidential and privileged information for the sole use of the designated recipient named above. Distribution, reproduction or any other use of this transmission by any party other than the intended recipient is prohibited. If you are not the intended recipient please contact the sender and delete all copies.
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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Vytas Bindokas, Ph.D.
Research Assoc. (Assoc. Prof.),
Director, BSD Light Microscopy Core Facility
phone: [phone]

      [address for letters ONLY (see shipping addr below):]
Dept Pharmacol Physiol Sci MC0926
947 E 58th Street
The University of Chicago
Chicago IL 60637
Room Abbott 129


shipping address (main KCBD site):
V. Bindokas
900 E 57th Street
KCBD room 1250, Microscopy Core
The University of Chicago
Chicago IL 60637


email [hidden email]
web site for LMCF:
http://digital.uchicago.edu/index.html

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