bUnwarpJ for Z-stack fluorescence image

[LIM Soon Yew John (IMB)]

Dear All,

Can I please check if bUnwarpJ can be used to correct for chromatic aberration for 2 channels Z-stack super-resolution fluorescence images? Will the transformation cause any misalignment in Z axis?

Please note the transformation file for bUnwarpJ is created by using super-resolution images from 0.1 um multi-wavelength beads.

Best Regards,
John

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[Ignacio Arganda-Carreras-2]

Hello John,

bUnwarpJ can correct misalignments in 2D but remember it's not a 3D
registratoin method so it won't affect the Z axis of your data.

What do you exactly need to register? A sequence of 2D images?

ignacio

On Wed, Apr 1, 2015 at 4:34 AM, LIM Soon Yew John (IMB) <
[hidden email]> wrote:

> Dear All,
>
> Can I please check if bUnwarpJ can be used to correct for chromatic
> aberration for 2 channels Z-stack super-resolution fluorescence images?
> Will the transformation cause any misalignment in Z axis?
>
> Please note the transformation file for bUnwarpJ is created by using
> super-resolution images from 0.1 um multi-wavelength beads.
>
> Best Regards,
> John
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Ignacio Arganda-Carreras, Ph.D.
Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 2
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

Tel : [phone] - fax : [phone]
Website: http://sites.google.com/site/iargandacarreras/
<http://biocomp.cnb.csic.es/~iarganda/index_EN.html>

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