I have reinstalled IMagej and upgraded to the newest version.
Bacgkround subtraction now works in green channel but not in blue. (using 50 as
radius and white background option unchecked)
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shows the image used. (i uploaded the tiff, but somhows it became png. )
Open the tiff in IMAGEJ Imgae> Color> RGBsplit> then take a historgam before
and after the background subtraction.
Upon examining the histogram, you will see that Green channel mean is lower
after background subtraction. But blue channel mean is identical.
Am I not doing this right?