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Hi,
I have a Macro that takes a folder of .LSM Zeiss confocal stacks with varying numbers of channels (between 2 and 4), opens them using the Bio-Formats Importer spliting the channels and saving them as .nrrd, .tif (16bit) and .tif (8bit) in separate folders.
What I want is to add to this Macro so that it also takes individual channels in the 8bit-tif folder and merges the channels then saves them in a new folder called merged.
The filenames in the 8bit-tif folder are in this format:
BADS01_experiment1_01.tif
BADS01_experiment1_02.tif
BADS02_experiment1_01.tif
BADS02_experiment1_02.tif
BADS02_experiment1_03.tif
BADS03_experiment1_01.tif
BADS03_experiment1_02.tif
BADS03_experiment1_03.tif
BADS03_experiment1_04.tif
Where BADS01, BADS02 and BADS03 are separate samples and the channels are denoted by the two digits before the .tif.
So I need a macro to merge the channels for BADS01, BADS02 and BADS03.
Can anyone help?
Thanks!
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