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Hi all,
I'm a newbie concerning imageJ/FIJI, especially when it comes to batch runs and computers in general (i work with mac). I've written following macro (see below) and it works fine when running it individually.
setBatchMode(false);
run("Stack to Images");
var s = getTitle();
rename(s + "dapi");
run("Put Behind [tab]");
run("Close");
run("Close");
selectWindow(s + "dapi");
setForegroundColor(0, 0, 0);
setBackgroundColor(0, 0, 0);
setThreshold(10, 255);
run("Create Selection");
run("Enlarge...", "enlarge=50 pixel");
run("Add to Manager");
roiManager("Select", 0);
roiManager("Rename", "dapi auto");
roiManager("Deselect");
roiManager("Save", "/Users/violettecoppensimac/Documents/130218 VD Tet VEGF/ROI Tet Vegf/ROI " + s + " dapi auto.zip");
roiManager("Delete");
selectWindow(s + "dapi");
run("Close");
However, when running a batch process I encounter 2 problems:
1) when running in batchmode true, the command is executed on the wrong image. this i can work around by processing in false mode
2) when running in false mode, the macro finishes the first image, but then returns following exception:
Fiji/ImageJ 1.47n; Java 1.6.0_43 [64-bit]; Mac OS X 10.8.3; 1936MB of 3269MB (59%)
java.lang.reflect.InvocationTargetException
at sun.reflect.GeneratedMethodAccessor4.invoke(Unknown Source)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:25)
at java.lang.reflect.Method.invoke(Method.java:597)
at ij.Command.runPlugIn(Command.java:148)
at ij.Command.runCommand(Command.java:97)
at ij.Executer.run(Executer.java:64)
at java.lang.Thread.run(Thread.java:680)
Caused by: java.lang.NullPointerException
at ij.gui.ImageWindow.close(ImageWindow.java:349)
at ij.gui.StackWindow.close(StackWindow.java:169)
at ij.ImagePlus.close(ImagePlus.java:338)
at ij.plugin.BatchProcesser.processFolder(BatchProcesser.java:195)
at ij.plugin.BatchProcesser.run(BatchProcesser.java:91)
at ij.IJ.runPlugIn(IJ.java:166)
... 7 more
Can somebody please explain in very simple terms (I'm a computer dummy ;) ) how this happens and how I can solve this?
many thanks!
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