bioformat autoscale is greyed out

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bioformat autoscale is greyed out

jarvisb2
I am trying to import .zgi files from a Zeiss microscope. The files come in and present as black boxes. I can manually adjust each color in the 4 color images with ImageJ brightness panel.  Autoscale on the Bioformat import window is greyed out. Any thought or suggestions for a fix?
 imageJ build 1.51g  Java 1.8 on Mac running OX X El Capitan  10.11.6 2.2gHz Intel Core i7
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Re: bioformat autoscale is greyed out

ctrueden
Hi Brenda,

> I am trying to import .zgi files from a Zeiss microscope.

I hope you mean .zvi or .czi. I have not heard of .zgi, and would be
saddened to hear that Zeiss has invented yet another file format... ;-)

> Autoscale on the Bioformat import window is greyed out.

Probably because you have the "virtual stack" option checked?

We never implemented that option for virtual stacks. It would only
autoscale your first plane anyway, because all the pixel values have not
yet been read (they are read on demand as you move the sliders).

Regards,
Curtis

--
Curtis Rueden
LOCI software architect - http://loci.wisc.edu/software
ImageJ2 lead, Fiji maintainer - http://imagej.net/User:Rueden
Did you know ImageJ has a forum? http://forum.imagej.net/


On Wed, Sep 28, 2016 at 3:47 PM, jarvisb2 <[hidden email]>
wrote:

> I am trying to import .zgi files from a Zeiss microscope. The files come in
> and present as black boxes. I can manually adjust each color in the 4 color
> images with ImageJ brightness panel.  Autoscale on the Bioformat import
> window is greyed out. Any thought or suggestions for a fix?
>  imageJ build 1.51g  Java 1.8 on Mac running OX X El Capitan  10.11.6
> 2.2gHz
> Intel Core i7
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.
> com/bioformat-autoscale-is-greyed-out-tp5017249.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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Re: bioformat autoscale is greyed out

jarvisb2
Yes it was .czi.  And unchecking the virtual stack option fixed it. Thank you!

Brenda Jarvis
Wright Lab 
MRBIV 9415
615-343-8257



On Sep 28, 2016, at 10:16 PM, ctrueden [via ImageJ] <[hidden email]> wrote:

Hi Brenda,

> I am trying to import .zgi files from a Zeiss microscope.

I hope you mean .zvi or .czi. I have not heard of .zgi, and would be
saddened to hear that Zeiss has invented yet another file format... ;-)

> Autoscale on the Bioformat import window is greyed out.

Probably because you have the "virtual stack" option checked?

We never implemented that option for virtual stacks. It would only
autoscale your first plane anyway, because all the pixel values have not
yet been read (they are read on demand as you move the sliders).

Regards,
Curtis

--
Curtis Rueden
LOCI software architect - http://loci.wisc.edu/software
ImageJ2 lead, Fiji maintainer - http://imagej.net/User:Rueden
Did you know ImageJ has a forum? http://forum.imagej.net/


On Wed, Sep 28, 2016 at 3:47 PM, jarvisb2 <<a href="x-msg://10/user/SendEmail.jtp?type=node&amp;node=5017255&amp;i=0" target="_top" rel="nofollow" link="external" class="">[hidden email]>
wrote:

> I am trying to import .zgi files from a Zeiss microscope. The files come in
> and present as black boxes. I can manually adjust each color in the 4 color
> images with ImageJ brightness panel.  Autoscale on the Bioformat import
> window is greyed out. Any thought or suggestions for a fix?
>  imageJ build 1.51g  Java 1.8 on Mac running OX X El Capitan  10.11.6
> 2.2gHz
> Intel Core i7
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.
> com/bioformat-autoscale-is-greyed-out-tp5017249.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html



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