Paul,
You need to calibrate your images. This could be done in one of two
ways: (i) manually scale an image using Analyse - Set Scale, or, (ii) by
having Microscope_Scale plugin compiled with the calibration table for
your imaging system and running it for each image. Either of these
methods can be adapted into a macro. Also, if all images are at the same
scale, you could also use the Global option when scaling the first image
and the same scale will be applied to all images. AFAIK, the scaling
information gets saved with the image file only when saved after
scaling, in TIFF format.
HTH,
Divakar
On Wednesday 27 April 2011 10:57 PM, paul_rostand wrote:
> Hello dear imagej users,
>
> I am an absolute beginner,I just knew about the software 2 days ago. I have a
> file with 1000 grayscale pictures, some with a black spot in the middle of a
> bright ring and somewithout. I am trying to sort automatically the pictures and
> determine in the same time the width and the position of the minimum of the
> black spot.
> I draw a rectangular and used the dynamic profiler.But in the profiler plot the
> width of the hole is always given in pixels.
>
> 1-Is there any way to acces the measured width and save it automatically in an
> array so that I can use it as criteria to sort the picture in a macro?
> 2-is there any way to convert directly the values in cm ?
>
> thank you in advance
> Paul Rostand
>
> --
> View this message in context:
http://imagej.588099.n2.nabble.com/convert-a-distance-in-pixel-to-a-distance-in-length-inch-or-cm-tp6310402p6310402.html> Sent from the ImageJ mailing list archive at Nabble.com.
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