convert video stream in stack

[Daniel James White]

Hi Felipe,

you can use something like

ffmpeg  (or the GUI version ffmpegX ona mac http://www.ffmpegx.com/download.html)

to convert the .vob from the DVD into a non compredded .avi  or other non lossy compressed format
which then imageJ can read as a timeframe stack.

But, this file will be very large, and you might want to do it in the small chunks you really want,
or else you need a powerful computer with tens of GB or RAM.

Dan

Begin forwarded message:

>
> Date:    Mon, 2 Aug 2010 20:45:39 -0300
> From:    Felipe Franco <[hidden email]>
> Subject: convert video stream in stack
>
>          Guys,
>
>          I m working in a molecular imaging project and we need to measure the fluorescence of organs in diferents intervals, not in a specific time. I just cannot find any software that is able to take an video stream ( working in set intervals ) and transforme it in a stack .tiff. Do you guys have some sugestion? Do you guys  think that if i convert my .vob file ( dvd video ) in quick time and then into a stack will lose some quality?
>
>           I need to point out that this video has about 1 hour in high quality and I intent to take some intervals between 5 and 15 minutes.
>
>           Thanks
>  

Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

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