Hi Felipe,
you can use something like
ffmpeg (or the GUI version ffmpegX ona mac
http://www.ffmpegx.com/download.html)
to convert the .vob from the DVD into a non compredded .avi or other non lossy compressed format
which then imageJ can read as a timeframe stack.
But, this file will be very large, and you might want to do it in the small chunks you really want,
or else you need a powerful computer with tens of GB or RAM.
Dan
Begin forwarded message:
>
> Date: Mon, 2 Aug 2010 20:45:39 -0300
> From: Felipe Franco <
[hidden email]>
> Subject: convert video stream in stack
>
> Guys,
>
> I m working in a molecular imaging project and we need to measure the fluorescence of organs in diferents intervals, not in a specific time. I just cannot find any software that is able to take an video stream ( working in set intervals ) and transforme it in a stack .tiff. Do you guys have some sugestion? Do you guys think that if i convert my .vob file ( dvd video ) in quick time and then into a stack will lose some quality?
>
> I need to point out that this video has about 1 hour in high quality and I intent to take some intervals between 5 and 15 minutes.
>
> Thanks
>
Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany
+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
http://www.bioimagexd.net BioImageXD
http://pacific.mpi-cbg.de Fiji - is just ImageJ (Batteries Included)
http://www.chalkie.org.uk Dan's Homepages
https://ifn.mpi-cbg.de Dresden Imaging Facility Network
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )
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