count two types of cells

[thuraya]

Hello,
 plz i need your help,, i need to count two types of cells the red cells pointed to by arrows and clear cells pointed to by arrowheads

[Peter Haub]

Hi thuraya,
still looking for an answer?

Use the Color Deconvolution plugin

http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

to separate your stains.
After that you can use/try common cell counting techniques (e.g. Thresholding, Particel Analyzer etc.

Regards,
Peter


On 17.08.2012 17:10, thuraya wrote:

> Hello,
>   plz i need your help,, i need to count two types of cells the red cells
> pointed to by arrows and clear cells pointed to by arrowheads
> http://imagej.1557.n6.nabble.com/file/n4999809/IMG_0654.jpg
>
>
>
> --
> View this message in context: http://imagej.1557.n6.nabble.com/count-two-types-of-cells-tp4999809.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[thuraya]

thanks alot for your reply.. i could count the purple cells using color Deconvolution plugin but again i couldn't count the white one.. please if you can help me to know how