counting particles within an are

[gilsimone]

Hi
Any advice on counting particles within a specific are? I can count the particles within the whole image
but having a hard time to restrict in to a certain area
Thank you

[ed3203]

if you are using the analyse; analyse particles function you can simply create a box using the "create box button" or by using the following macro:

makeRectangle(292, 363, 73, 75);

the threshold will set to the box properties and the analysis will work from that.

[gilsimone]

Thanks.

What about if I want to trace a contour around an area (which is a blob of color) and then count the particles within that area?

On Wed, Feb 2, 2011 at 12:57 PM, ed3203 [via ImageJ] <[hidden email]> wrote:

if you are using the analyse; analyse particles function you can simply create a box using the "create box button" or by using the following macro:

makeRectangle(292, 363, 73, 75);

the threshold will set to the box properties and the analysis will work from that.

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