densitometry

[Miguel Valvano]

I would like to use imageJ for densitometric analysis of western blots.
Does anybody have a writtne protocol. I find it confusion using the
menus in the gel analyzer.
Thanks
 
 
Miguel A. Valvano, M.D.
Professor and Department Chair
Canada Research Chair in Infectious Diseases and Microbial
Pathogenesis
Department of Microbiology and Immunology, University of Western
Ontario
London Ontario, N6A 5C1
voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
Assistant: Ms. Evelynne Ansara ([hidden email]);
519-661-2111 #81436

[Jonathan Hilmer]

I have written a guide for ImageJ densitometry, but it isn't really a
straightforward how-to type manual.  The method also relies upon
outside software (Photoshop or an equivalent) for convenience, uses
modified ImageJ GelAnalyzer code, and assumes the user has a decent
background in processing images in ImageJ.  The benefit from these
inconveniences is that the final result is more accurate than other
methods.


Jonathan Hilmer
Bothner Research Group
Montana State University



On 8/16/06, Miguel Valvano <[hidden email]> wrote:

> I would like to use imageJ for densitometric analysis of western blots.
> Does anybody have a writtne protocol. I find it confusion using the
> menus in the gel analyzer.
> Thanks
>
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
> Assistant: Ms. Evelynne Ansara ([hidden email]);
> 519-661-2111 #81436

[Miguel Valvano]

Thank you. I would like to give it a try. Could you mind sending me a
copy of your guide?
Thanks
M
 
Miguel A. Valvano, M.D.
Professor and Department Chair
Canada Research Chair in Infectious Diseases and Microbial
Pathogenesis
Department of Microbiology and Immunology, University of Western
Ontario
London Ontario, N6A 5C1
voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
Assistant: Ms. Evelynne Ansara ([hidden email]);
519-661-2111 #81436

>>> [hidden email] 08/16/06 4:37 pm >>>

I have written a guide for ImageJ densitometry, but it isn't really a
straightforward how-to type manual.  The method also relies upon
outside software (Photoshop or an equivalent) for convenience, uses
modified ImageJ GelAnalyzer code, and assumes the user has a decent
background in processing images in ImageJ.  The benefit from these
inconveniences is that the final result is more accurate than other
methods.


Jonathan Hilmer
Bothner Research Group
Montana State University



On 8/16/06, Miguel Valvano <[hidden email]> wrote:
> I would like to use imageJ for densitometric analysis of western
blots.

> Does anybody have a writtne protocol. I find it confusion using the
> menus in the gel analyzer.
> Thanks
>
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax [phone];

http://publish.uwo.ca/~mvalvano
> Assistant: Ms. Evelynne Ansara ([hidden email]);
> 519-661-2111 #81436

[dksamuel]

I would also like to receive a copy, thanks Samuel Virologist India

On 8/17/06, Miguel Valvano <[hidden email]> wrote:

>
> Thank you. I would like to give it a try. Could you mind sending me a
> copy of your guide?
> Thanks
> M
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
> Assistant: Ms. Evelynne Ansara ([hidden email]);
> 519-661-2111 #81436
>
> >>> [hidden email] 08/16/06 4:37 pm >>>
>
> I have written a guide for ImageJ densitometry, but it isn't really a
> straightforward how-to type manual.  The method also relies upon
> outside software (Photoshop or an equivalent) for convenience, uses
> modified ImageJ GelAnalyzer code, and assumes the user has a decent
> background in processing images in ImageJ.  The benefit from these
> inconveniences is that the final result is more accurate than other
> methods.
>
>
> Jonathan Hilmer
> Bothner Research Group
> Montana State University
>
>
>
> On 8/16/06, Miguel Valvano <[hidden email]> wrote:
> > I would like to use imageJ for densitometric analysis of western
> blots.
> > Does anybody have a writtne protocol. I find it confusion using the
> > menus in the gel analyzer.
> > Thanks
> >
> >
> > Miguel A. Valvano, M.D.
> > Professor and Department Chair
> > Canada Research Chair in Infectious Diseases and Microbial
> > Pathogenesis
> > Department of Microbiology and Immunology, University of Western
> > Ontario
> > London Ontario, N6A 5C1
> > voice 519-661-3436; fax [phone];
> http://publish.uwo.ca/~mvalvano
> > Assistant: Ms. Evelynne Ansara ([hidden email]);
> > 519-661-2111 #81436
>

[Palpandi Raja]

I would also like to have a copy of the guide for densitometric analysis.
I am intending to use it for SDS-PAGE analysis. Thanks

Regards,
Palpandi

--
R. Palpandi Raja, 112B Bioprocess Eng Lab, Chem Eng Dept, IIT Bombay,
Powai, Mumbai, India. Tel:[phone] Fax:[phone]

[Marco Coan]

Can you send it to me too? I often work with gel elctrophoresis and your
guide could be interesting to me. Thank you.

Marco Coan
Food Science Department
Udine University, Italy

2006/8/16, Jonathan Hilmer <[hidden email]>:

>
> I have written a guide for ImageJ densitometry, but it isn't really a
> straightforward how-to type manual.  The method also relies upon
> outside software (Photoshop or an equivalent) for convenience, uses
> modified ImageJ GelAnalyzer code, and assumes the user has a decent
> background in processing images in ImageJ.  The benefit from these
> inconveniences is that the final result is more accurate than other
> methods.
>
>
> Jonathan Hilmer
> Bothner Research Group
> Montana State University
>
>
>
> On 8/16/06, Miguel Valvano <[hidden email]> wrote:
> > I would like to use imageJ for densitometric analysis of western blots.
> > Does anybody have a writtne protocol. I find it confusion using the
> > menus in the gel analyzer.
> > Thanks
> >
> >
> > Miguel A. Valvano, M.D.
> > Professor and Department Chair
> > Canada Research Chair in Infectious Diseases and Microbial
> > Pathogenesis
> > Department of Microbiology and Immunology, University of Western
> > Ontario
> > London Ontario, N6A 5C1
> > voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
> > Assistant: Ms. Evelynne Ansara ([hidden email]);
> > 519-661-2111 #81436
>

--
Ai sensi del D.Lgs. 196/2003 si precisa che le informazioni contenute in
questo messaggio sono riservate ed a uso esclusivo del destinatario. Qualora
il messaggio in parola Le fosse pervenuto per errore, La invitiamo ad
eliminarlo senza copiarlo e a non inoltrarlo a terzi, dandocene gentilmente
comunicazione. Grazie.

Pursuant to Legislative Decree No. 196/2003, you are hereby informed that
this message contains confidential information intended only for the use of
the addressee. If you are not the addressee, and have received this
message by mistake, please delete it and immediately notify us. You may not
copy or disseminate this message to anyone. Thank you.

[Nas-5]

Could you also send a copy to me? Thank you.

Best
Nas

--- Marco Coan <[hidden email]> wrote:

> Can you send it to me too? I often work with gel
> elctrophoresis and your
> guide could be interesting to me. Thank you.
>
> Marco Coan
> Food Science Department
> Udine University, Italy
>
> 2006/8/16, Jonathan Hilmer <[hidden email]>:
> >
> > I have written a guide for ImageJ densitometry,
> but it isn't really a
> > straightforward how-to type manual.  The method
> also relies upon
> > outside software (Photoshop or an equivalent) for
> convenience, uses
> > modified ImageJ GelAnalyzer code, and assumes the
> user has a decent
> > background in processing images in ImageJ.  The
> benefit from these
> > inconveniences is that the final result is more
> accurate than other
> > methods.
> >
> >
> > Jonathan Hilmer
> > Bothner Research Group
> > Montana State University
> >
> >
> >
> > On 8/16/06, Miguel Valvano
> <[hidden email]> wrote:
> > > I would like to use imageJ for densitometric
> analysis of western blots.
> > > Does anybody have a writtne protocol. I find it
> confusion using the
> > > menus in the gel analyzer.
> > > Thanks
> > >
> > >
> > > Miguel A. Valvano, M.D.
> > > Professor and Department Chair
> > > Canada Research Chair in Infectious Diseases and
> Microbial
> > > Pathogenesis
> > > Department of Microbiology and Immunology,
> University of Western
> > > Ontario
> > > London Ontario, N6A 5C1
> > > voice 519-661-3436; fax [phone];
> http://publish.uwo.ca/~mvalvano
> > > Assistant: Ms. Evelynne Ansara
> ([hidden email]);
> > > 519-661-2111 #81436
> >
>
>
>
> --
> Ai sensi del D.Lgs. 196/2003 si precisa che le
> informazioni contenute in
> questo messaggio sono riservate ed a uso esclusivo
> del destinatario. Qualora
> il messaggio in parola Le fosse pervenuto per
> errore, La invitiamo ad
> eliminarlo senza copiarlo e a non inoltrarlo a
> terzi, dandocene gentilmente
> comunicazione. Grazie.
>
> Pursuant to Legislative Decree No. 196/2003, you are
> hereby informed that
> this message contains confidential information
> intended only for the use of
> the addressee. If you are not the addressee, and
> have received this
> message by mistake, please delete it and immediately
> notify us. You may not
> copy or disseminate this message to anyone. Thank
> you.
>

__________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around
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[Andrea-22]

Could you also send me a copy?
Thank you!!.

Best

Dr. Andrea Rabellino,
Istituto FIRC di Oncologia Molecolare,
Dipartimento di Medicina Sperimentale,
Università degli Studi di Genova,
Via De Toni 14,
Genova

----- Original Message -----
From: "Nas" <[hidden email]>
To: <[hidden email]>
Sent: Thursday, August 17, 2006 3:45 PM
Subject: Re: densitometry

> Could you also send a copy to me? Thank you.
>
> Best
> Nas
>
> --- Marco Coan <[hidden email]> wrote:
>
>> Can you send it to me too? I often work with gel
>> elctrophoresis and your
>> guide could be interesting to me. Thank you.
>>
>> Marco Coan
>> Food Science Department
>> Udine University, Italy
>>
>> 2006/8/16, Jonathan Hilmer <[hidden email]>:
>> >
>> > I have written a guide for ImageJ densitometry,
>> but it isn't really a
>> > straightforward how-to type manual.  The method
>> also relies upon
>> > outside software (Photoshop or an equivalent) for
>> convenience, uses
>> > modified ImageJ GelAnalyzer code, and assumes the
>> user has a decent
>> > background in processing images in ImageJ.  The
>> benefit from these
>> > inconveniences is that the final result is more
>> accurate than other
>> > methods.
>> >
>> >
>> > Jonathan Hilmer
>> > Bothner Research Group
>> > Montana State University
>> >
>> >
>> >
>> > On 8/16/06, Miguel Valvano
>> <[hidden email]> wrote:
>> > > I would like to use imageJ for densitometric
>> analysis of western blots.
>> > > Does anybody have a writtne protocol. I find it
>> confusion using the
>> > > menus in the gel analyzer.
>> > > Thanks
>> > >
>> > >
>> > > Miguel A. Valvano, M.D.
>> > > Professor and Department Chair
>> > > Canada Research Chair in Infectious Diseases and
>> Microbial
>> > > Pathogenesis
>> > > Department of Microbiology and Immunology,
>> University of Western
>> > > Ontario
>> > > London Ontario, N6A 5C1
>> > > voice 519-661-3436; fax [phone];
>> http://publish.uwo.ca/~mvalvano
>> > > Assistant: Ms. Evelynne Ansara
>> ([hidden email]);
>> > > 519-661-2111 #81436
>> >
>>
>>
>>
>> --
>> Ai sensi del D.Lgs. 196/2003 si precisa che le
>> informazioni contenute in
>> questo messaggio sono riservate ed a uso esclusivo
>> del destinatario. Qualora
>> il messaggio in parola Le fosse pervenuto per
>> errore, La invitiamo ad
>> eliminarlo senza copiarlo e a non inoltrarlo a
>> terzi, dandocene gentilmente
>> comunicazione. Grazie.
>>
>> Pursuant to Legislative Decree No. 196/2003, you are
>> hereby informed that
>> this message contains confidential information
>> intended only for the use of
>> the addressee. If you are not the addressee, and
>> have received this
>> message by mistake, please delete it and immediately
>> notify us. You may not
>> copy or disseminate this message to anyone. Thank
>> you.
>>
>
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam?  Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com 

[Zafer C. Cehreli]

I would also appreciate one copy. Many thanks in
advance!

Zafer

--- Andrea <[hidden email]> wrote:

> Could you also send me a copy?
> Thank you!!.
>
> Best
>
> Dr. Andrea Rabellino,
> Istituto FIRC di Oncologia Molecolare,
> Dipartimento di Medicina Sperimentale,
> Università degli Studi di Genova,
> Via De Toni 14,
> Genova
>
> ----- Original Message -----
> From: "Nas" <[hidden email]>
> To: <[hidden email]>
> Sent: Thursday, August 17, 2006 3:45 PM
> Subject: Re: densitometry
>
>
> > Could you also send a copy to me? Thank you.
> >
> > Best
> > Nas
> >
> > --- Marco Coan <[hidden email]> wrote:
> >
> >> Can you send it to me too? I often work with gel
> >> elctrophoresis and your
> >> guide could be interesting to me. Thank you.
> >>
> >> Marco Coan
> >> Food Science Department
> >> Udine University, Italy
> >>
> >> 2006/8/16, Jonathan Hilmer <[hidden email]>:
> >> >
> >> > I have written a guide for ImageJ densitometry,
> >> but it isn't really a
> >> > straightforward how-to type manual.  The method
> >> also relies upon
> >> > outside software (Photoshop or an equivalent)
> for
> >> convenience, uses
> >> > modified ImageJ GelAnalyzer code, and assumes
> the
> >> user has a decent
> >> > background in processing images in ImageJ.  The
> >> benefit from these
> >> > inconveniences is that the final result is more
> >> accurate than other
> >> > methods.
> >> >
> >> >
> >> > Jonathan Hilmer
> >> > Bothner Research Group
> >> > Montana State University
> >> >
> >> >
> >> >
> >> > On 8/16/06, Miguel Valvano
> >> <[hidden email]> wrote:
> >> > > I would like to use imageJ for densitometric
> >> analysis of western blots.
> >> > > Does anybody have a writtne protocol. I find
> it
> >> confusion using the
> >> > > menus in the gel analyzer.
> >> > > Thanks
> >> > >
> >> > >
> >> > > Miguel A. Valvano, M.D.
> >> > > Professor and Department Chair
> >> > > Canada Research Chair in Infectious Diseases
> and
> >> Microbial
> >> > > Pathogenesis
> >> > > Department of Microbiology and Immunology,
> >> University of Western
> >> > > Ontario
> >> > > London Ontario, N6A 5C1
> >> > > voice 519-661-3436; fax [phone];
> >> http://publish.uwo.ca/~mvalvano
> >> > > Assistant: Ms. Evelynne Ansara
> >> ([hidden email]);
> >> > > 519-661-2111 #81436
> >> >
> >>
> >>
> >>
> >> --
> >> Ai sensi del D.Lgs. 196/2003 si precisa che le
> >> informazioni contenute in
> >> questo messaggio sono riservate ed a uso
> esclusivo
> >> del destinatario. Qualora
> >> il messaggio in parola Le fosse pervenuto per
> >> errore, La invitiamo ad
> >> eliminarlo senza copiarlo e a non inoltrarlo a
> >> terzi, dandocene gentilmente
> >> comunicazione. Grazie.
> >>
> >> Pursuant to Legislative Decree No. 196/2003, you
> are
> >> hereby informed that
> >> this message contains confidential information
> >> intended only for the use of
> >> the addressee. If you are not the addressee, and
> >> have received this
> >> message by mistake, please delete it and
> immediately
> >> notify us. You may not
> >> copy or disseminate this message to anyone. Thank
> >> you.
> >>
> >
> >
> > __________________________________________________
> > Do You Yahoo!?
> > Tired of spam?  Yahoo! Mail has the best spam
> protection around
> > http://mail.yahoo.com 
>

__________________________________________________
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Tired of spam?  Yahoo! Mail has the best spam protection around
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[Przemko]

Could you just post a copy somewhere on the net?
Przemko

--
         Przemko Tylzanowski Ph.D.
         LSD & Joint, box 813-GREEN
         O & N
         University of Leuven
         Herestraat 49
         3000 Leuven
         Belgium
         
         phone: [phone]
         fax  : [phone]
         


Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm

[Volker Baecker]

If you're willing to put it online, I think the ImageJ documentation
wiki would be a good place.
http://imagejdocu.tudor.lu/imagej-documenation-wiki/
Volker

Przemko a écrit :
> Could you just post a copy somewhere on the net?
> Przemko
>

--
passerelle antivirus du campus CNRS de Montpellier
--

[Sarwar, Rizwan]

Jonathan

Can I please also have a copy too for SDS-PAGE analysis

Tx

Rizwan Sarwar
Physiological Genomics & Medicine
Imperial College London

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Jonathan Hilmer
Sent: 16 August 2006 21:37
To: [hidden email]
Subject: Re: densitometry

I have written a guide for ImageJ densitometry, but it isn't really a
straightforward how-to type manual.  The method also relies upon
outside software (Photoshop or an equivalent) for convenience, uses
modified ImageJ GelAnalyzer code, and assumes the user has a decent
background in processing images in ImageJ.  The benefit from these
inconveniences is that the final result is more accurate than other
methods.


Jonathan Hilmer
Bothner Research Group
Montana State University



On 8/16/06, Miguel Valvano <[hidden email]> wrote:
> I would like to use imageJ for densitometric analysis of western
blots.

> Does anybody have a writtne protocol. I find it confusion using the
> menus in the gel analyzer.
> Thanks
>
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
> Assistant: Ms. Evelynne Ansara ([hidden email]);
> 519-661-2111 #81436

[Ruy]

Could you also send me a copy?

Thank yoy

Ruy Jaeger
Cell Biology
University of São Paulo, Brazil

On 17/8/06 11:23 AM, "Andrea" <[hidden email]> wrote:

> Could you also send me a copy?
> Thank you!!.
>
> Best
>
> Dr. Andrea Rabellino,
> Istituto FIRC di Oncologia Molecolare,
> Dipartimento di Medicina Sperimentale,
> Università degli Studi di Genova,
> Via De Toni 14,
> Genova
>
> ----- Original Message -----
> From: "Nas" <[hidden email]>
> To: <[hidden email]>
> Sent: Thursday, August 17, 2006 3:45 PM
> Subject: Re: densitometry
>
>
>> Could you also send a copy to me? Thank you.
>>
>> Best
>> Nas
>>
>> --- Marco Coan <[hidden email]> wrote:
>>
>>> Can you send it to me too? I often work with gel
>>> elctrophoresis and your
>>> guide could be interesting to me. Thank you.
>>>
>>> Marco Coan
>>> Food Science Department
>>> Udine University, Italy
>>>
>>> 2006/8/16, Jonathan Hilmer <[hidden email]>:
>>>>
>>>> I have written a guide for ImageJ densitometry,
>>> but it isn't really a
>>>> straightforward how-to type manual.  The method
>>> also relies upon
>>>> outside software (Photoshop or an equivalent) for
>>> convenience, uses
>>>> modified ImageJ GelAnalyzer code, and assumes the
>>> user has a decent
>>>> background in processing images in ImageJ.  The
>>> benefit from these
>>>> inconveniences is that the final result is more
>>> accurate than other
>>>> methods.
>>>>
>>>>
>>>> Jonathan Hilmer
>>>> Bothner Research Group
>>>> Montana State University
>>>>
>>>>
>>>>
>>>> On 8/16/06, Miguel Valvano
>>> <[hidden email]> wrote:
>>>>> I would like to use imageJ for densitometric
>>> analysis of western blots.
>>>>> Does anybody have a writtne protocol. I find it
>>> confusion using the
>>>>> menus in the gel analyzer.
>>>>> Thanks
>>>>>
>>>>>
>>>>> Miguel A. Valvano, M.D.
>>>>> Professor and Department Chair
>>>>> Canada Research Chair in Infectious Diseases and
>>> Microbial
>>>>> Pathogenesis
>>>>> Department of Microbiology and Immunology,
>>> University of Western
>>>>> Ontario
>>>>> London Ontario, N6A 5C1
>>>>> voice 519-661-3436; fax [phone];
>>> http://publish.uwo.ca/~mvalvano
>>>>> Assistant: Ms. Evelynne Ansara
>>> ([hidden email]);
>>>>> 519-661-2111 #81436
>>>>
>>>
>>>
>>>
>>> --
>>> Ai sensi del D.Lgs. 196/2003 si precisa che le
>>> informazioni contenute in
>>> questo messaggio sono riservate ed a uso esclusivo
>>> del destinatario. Qualora
>>> il messaggio in parola Le fosse pervenuto per
>>> errore, La invitiamo ad
>>> eliminarlo senza copiarlo e a non inoltrarlo a
>>> terzi, dandocene gentilmente
>>> comunicazione. Grazie.
>>>
>>> Pursuant to Legislative Decree No. 196/2003, you are
>>> hereby informed that
>>> this message contains confidential information
>>> intended only for the use of
>>> the addressee. If you are not the addressee, and
>>> have received this
>>> message by mistake, please delete it and immediately
>>> notify us. You may not
>>> copy or disseminate this message to anyone. Thank
>>> you.
>>>
>>
>>
>> __________________________________________________
>> Do You Yahoo!?
>> Tired of spam?  Yahoo! Mail has the best spam protection around
>> http://mail.yahoo.com
>

 ========================================================================
                   Dr. Ruy G. Jaeger, DDS, MSD, PhD

 University of Sao Paulo            Institute of Biomedical Sciences
             Department of Cell and Developmental Biology
 Av. Prof. Lineu Prestes 1524 sala 405            Sao Paulo SP 05508-900
 Phone    [phone]                         FAX    [phone]
                                BRAZIL
          website: http://www.icb.usp.br/~rgjaeger/index.html
 ========================================================================

[Wen, Kuo-Kuang]

Could you also send me a copy about the densitometry protocol?

Thanks!!

Kuo-Kuang Wen

Department of Biochemistry
University of Iowa
Iowa City, IA 52242

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Ruy
Sent: Thursday, August 17, 2006 2:15 PM
To: [hidden email]
Subject: Re: densitometry

Could you also send me a copy?

Thank yoy

Ruy Jaeger
Cell Biology
University of São Paulo, Brazil

On 17/8/06 11:23 AM, "Andrea" <[hidden email]> wrote:

> Could you also send me a copy?
> Thank you!!.
>
> Best
>
> Dr. Andrea Rabellino,
> Istituto FIRC di Oncologia Molecolare,
> Dipartimento di Medicina Sperimentale,
> Università degli Studi di Genova,
> Via De Toni 14,
> Genova
>
> ----- Original Message -----
> From: "Nas" <[hidden email]>
> To: <[hidden email]>
> Sent: Thursday, August 17, 2006 3:45 PM
> Subject: Re: densitometry
>
>
>> Could you also send a copy to me? Thank you.
>>
>> Best
>> Nas
>>
>> --- Marco Coan <[hidden email]> wrote:
>>
>>> Can you send it to me too? I often work with gel
>>> elctrophoresis and your
>>> guide could be interesting to me. Thank you.
>>>
>>> Marco Coan
>>> Food Science Department
>>> Udine University, Italy
>>>
>>> 2006/8/16, Jonathan Hilmer <[hidden email]>:
>>>>
>>>> I have written a guide for ImageJ densitometry,
>>> but it isn't really a
>>>> straightforward how-to type manual.  The method
>>> also relies upon
>>>> outside software (Photoshop or an equivalent) for
>>> convenience, uses
>>>> modified ImageJ GelAnalyzer code, and assumes the
>>> user has a decent
>>>> background in processing images in ImageJ.  The
>>> benefit from these
>>>> inconveniences is that the final result is more
>>> accurate than other
>>>> methods.
>>>>
>>>>
>>>> Jonathan Hilmer
>>>> Bothner Research Group
>>>> Montana State University
>>>>
>>>>
>>>>
>>>> On 8/16/06, Miguel Valvano
>>> <[hidden email]> wrote:
>>>>> I would like to use imageJ for densitometric
>>> analysis of western blots.
>>>>> Does anybody have a writtne protocol. I find it
>>> confusion using the
>>>>> menus in the gel analyzer.
>>>>> Thanks
>>>>>
>>>>>
>>>>> Miguel A. Valvano, M.D.
>>>>> Professor and Department Chair
>>>>> Canada Research Chair in Infectious Diseases and
>>> Microbial
>>>>> Pathogenesis
>>>>> Department of Microbiology and Immunology,
>>> University of Western
>>>>> Ontario
>>>>> London Ontario, N6A 5C1
>>>>> voice 519-661-3436; fax [phone];
>>> http://publish.uwo.ca/~mvalvano
>>>>> Assistant: Ms. Evelynne Ansara
>>> ([hidden email]);
>>>>> 519-661-2111 #81436
>>>>
>>>
>>>
>>>
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 ========================================================================
                   Dr. Ruy G. Jaeger, DDS, MSD, PhD

 University of Sao Paulo            Institute of Biomedical Sciences
             Department of Cell and Developmental Biology
 Av. Prof. Lineu Prestes 1524 sala 405            Sao Paulo SP 05508-900
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[Diego De Stefani]

On Friday 18 August 2006 18:38, Wen, Kuo-Kuang wrote:
> Could you also send me a copy about the densitometry protocol?

Can I have one copy too?

[ctrueden]

Hi,

No fewer than a dozen people have now individually requested copies of this
(to the entire ImageJ list). Mr. Hilmer has not even said publically that he
would make the guide available. If you *must* request a copy, please email
him directly instead of cluttering the list with this any further.

Thanks,
Curtis

On 8/22/06, Diego De Stefani <[hidden email]> wrote:
>
> On Friday 18 August 2006 18:38, Wen, Kuo-Kuang wrote:
> > Could you also send me a copy about the densitometry protocol?
>
> Can I have one copy too?
>

[Jonathan Hilmer]

In the last week I've received a lot of interest in my crude guide,
but I would prefer not to have my incomplete tutorial serve as the
foundation for such an important topic.  Surely we can come up with
something better, and such a discussion would be more productive than
a one-sided distribution of my thoughts on the matter.  To this end,
I'll be creating an entry in the ImageJ wiki as time permits.  If
anyone has specific questions, let me know and I'll try to address
them.

Jonathan Hilmer
Bothner Research Group
Montana State University


On 8/16/06, Miguel Valvano <[hidden email]> wrote:

> I would like to use imageJ for densitometric analysis of western blots.
> Does anybody have a writtne protocol. I find it confusion using the
> menus in the gel analyzer.
> Thanks
>
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
> Assistant: Ms. Evelynne Ansara ([hidden email]);
> 519-661-2111 #81436
>