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Hi,
May I ask for help?
I am trying to open my confocal .lif file in image J and would like to get a view of the individual channels as well as a merged composition.
I managed somehow to get this (although I think I am doing it somewhat complicated) but when I compare the stacks in Image J and the actual .lif file at the microsope the colors appear much weaker and in fact it is hard to see my specific signal any more.
Is there a way to get the proper colors as they were at the microsope. (As I have isotype controls and different cells I want to compare I don't think I should play with the color balance ect)
Thank you very much.
Kind regards,
lukas
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