drift correction in one color applied o a second color

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drift correction in one color applied o a second color

Jeff Spector-2
  I have a 2 colors stack that I would like to correct for stage drift. The
red channel are objects that are static, but the green channel are very
dynamic objects. I would like to correct for drift using ONLY the red
channel but have that correction applied to both channels. How might I go
about accomplishing this? It seems to me that most of the drift correction
plugins work on either both channels, or you have to split the stack up and
correct each channel individually. That doesn't work with the data I have
(the green channel is to dynamic and there are a lot of intensity changes)…

can someone please help..

thanks..

-Jeff

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Re: drift correction in one color applied o a second color

Peterbauer Thomas
*Jeff,
*

*Check Stephan Preibisch's registration plugin (menu entry
"Descriptor-based series registration 2D/3D+t" in Fiji). It lets you
choose the channel used for registration. There **are**also other,
though less convenient options involving splitting of channels and
applying registration coefficients from one stack to others (e.g., with
the Image Stabilizer plugin).*

*-Thomas *



On 2014-04-25 20:46, Jeff Spector wrote:

>    I have a 2 colors stack that I would like to correct for stage drift. The
> red channel are objects that are static, but the green channel are very
> dynamic objects. I would like to correct for drift using ONLY the red
> channel but have that correction applied to both channels. How might I go
> about accomplishing this? It seems to me that most of the drift correction
> plugins work on either both channels, or you have to split the stack up and
> correct each channel individually. That doesn't work with the data I have
> (the green channel is to dynamic and there are a lot of intensity changes)…
>
> can someone please help..
>
> thanks..
>
> -Jeff
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html


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