import ij.*;
import ij.text.*;
import ij.process.*;
import ij.gui.*;
import java.awt.*;
import ij.plugin.*;
import ij.plugin.filter.*;

public class Hemozoin_Analysis implements PlugIn {

	public void run(String arg) {
		ImagePlus image = IJ.getImage();
		IJ.run("Subtract Background...", "rolling=50 light");
		IJ.run("Split Channels", "");
		IJ.run("Threshold..."); 
		IJ.setThreshold(0, 170);  
		IJ.run("Convert to Mask", "");
		IJ.run("Close");
		IJ.run("Create Selection", "");
		IJ.run("Measure", "");
		IJ.run("Close");
		IJ.run("Threshold..."); 
		IJ.setThreshold(0, 130);  
		IJ.run("Convert to Mask", "");
		IJ.run("Close");
		IJ.run("Create Selection", "");
		IJ.run("Measure", "");	
 		WindowManager.putBehind();
		IJ.run("Close");
		IJ.run("Close");
				
	}
}